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Clinical Translation of Stimulated Raman Histology

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Biomedical Engineering Technologies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2393))

Abstract

Stimulated Raman histology (SRH) images are created by the label-free, nondestructive imaging of tissue using stimulated Raman scattering (SRS) microscopy. In a matter of seconds, these images provide real-time histologic information on biopsied tissue in the operating room. SRS microscopy uses two lasers (pump beam and Stokes beam) to amplify the Raman signal of specific chemical bonds found in macromolecules (lipids, proteins, and nucleic acids) in these tissues. The concentrations of these macromolecules are used to produce image contrast. These images are acquired and displayed using an imaging system with five main components: (1) fiber coupled microscope, (2) dual-wavelength fiber-laser module, (3) laser control module, (4) microscope control module, and (5) a computer. This manuscript details how to assemble the dual-wavelength fiber-laser module and how to generate an SRH image.

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Authors and Affiliations

  1. NYU Langone Neurosurgery Associates, New York, NY, USA

    Cordelia Orillac, Todd Hollon & Daniel A. Orringer

Authors
  1. Cordelia Orillac
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  2. Todd Hollon
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  3. Daniel A. Orringer
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Corresponding author

Correspondence to Daniel A. Orringer .

Editor information

Editors and Affiliations

  1. Division of Cancer Treatment and Diagnosis, National Cancer Institute, NIH, Bethesda, MD, USA

    Miguel R. Ossandon

  2. Division of Cancer Treatment and Diagnosis, National Cancer Institute, Rockville, MD, USA

    Houston Baker

  3. Division of Cancer Treatment and Diagnosis, National Cancer Institute, Rockville, MD, USA

    Avraham Rasooly

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Orillac, C., Hollon, T., Orringer, D.A. (2022). Clinical Translation of Stimulated Raman Histology. In: Ossandon, M.R., Baker, H., Rasooly, A. (eds) Biomedical Engineering Technologies. Methods in Molecular Biology, vol 2393. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1803-5_12

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  • DOI: https://doi.org/10.1007/978-1-0716-1803-5_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1802-8

  • Online ISBN: 978-1-0716-1803-5

  • eBook Packages: Springer Protocols

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