Abstract
Candida auris can persist for long periods on hospital surfaces and on the skin. C. auris has the ability to form drug-resistant biofilms, which can substantially impact on patient outcome. In comparison to Candida albicans, C. auris has a lower capacity to form biofilms in in vitro models and a higher capacity when tested on animal skin models. Intraspecies variation is shown to exist, with some clinical isolates having greater biofilm capabilities than others. There is a need for models that closely mimic the real niches where infection occurs on human patients. This protocol describes, in detail, a human skin model to study C. auris biofilm formation using catheterized and non-catheterized skin.
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Acknowledgments
This project was funded by a Wellcome Trust Strategic Award for Medical Mycology and Fungal Immunology (097377) and Medical Research Council Centre for Medical Mycology (MR/N006364/1), previously at the University of Aberdeen, now at the University of Exeter.
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Corzo-León, D.E., Mark, C., MacCallum, D.M., Munro, C.A. (2022). A Human Ex Vivo Skin Model to Study Candida auris Biofilms. In: Lorenz, A. (eds) Candida auris. Methods in Molecular Biology, vol 2517. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2417-3_21
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DOI: https://doi.org/10.1007/978-1-0716-2417-3_21
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