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Determination of Effects and Mechanisms of Action of Bacterial Amyloids on Antibiotic Resistance

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Bacterial Amyloids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2538))

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Abstract

Bacterial functional amyloids, apart from their many other functions, can influence the resistance of bacteria to antibiotics and other antibacterial agents. Mechanisms of modulation of susceptibility of bacterial cells to antimicrobials can be either indirect or direct. The former mechanisms are exemplified by the contribution of functional amyloids to biofilm formation, which may effectively prevent the penetration of various compounds into bacterial cells. The direct mechanisms include the effects of bacterial proteins revealing amyloid-like structures, like the C-terminal region of the Escherichia coli Hfq protein, on the expression of genes involved in antibiotic resistance. Therefore, in this paper, we describe methods by which effects and mechanisms of action of bacterial amyloids on antibiotic resistance can be studied. Assessment of formation of biofilms, determination of the efficiency of antibiotic resistance in solid and liquid media, and determination of the effects on gene expression at levels of mRNA abundance and stability and protein abundance are described.

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Author notes

  1. Krzysztof Kubiak, Lidia Gaffke, Karolina Pierzynowska, and Zuzanna Cyske contributed equally to this work.

Authors and Affiliations

  1. Department of Molecular Biology, University of Gdansk, Gdansk, Poland

    Krzysztof Kubiak, Lidia Gaffke, Karolina Pierzynowska, Zuzanna Cyske, Weronika Jaroszewicz & Grzegorz Węgrzyn

  2. Laboratory of Phage Therapy, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Gdansk, Poland

    Łukasz Grabowski, Katarzyna Kosznik-Kwaśnicka & Alicja Węgrzyn

  3. Department of Medical Microbiology, Faculty of Medicine, Medical University of Gdansk, Gdansk, Poland

    Katarzyna Kosznik-Kwaśnicka

Authors
  1. Krzysztof Kubiak
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  2. Lidia Gaffke
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  3. Karolina Pierzynowska
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  4. Zuzanna Cyske
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  5. Łukasz Grabowski
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  6. Katarzyna Kosznik-Kwaśnicka
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  7. Weronika Jaroszewicz
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  8. Alicja Węgrzyn
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  9. Grzegorz Węgrzyn
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Corresponding author

Correspondence to Grzegorz Węgrzyn .

Editor information

Editors and Affiliations

  1. CEA Saclay, Lab Léon Brillouin UMR12 CEA/CNRS, Gif sur Yvette Cedex, France

    Véronique Arluison

  2. Synchrotron SOLEIL, Gif-sur-Yvette Cedex, France

    Frank Wien

  3. Departamento de Biología, University of Chile, Santiago, Chile

    Andrés Marcoleta

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Kubiak, K. et al. (2022). Determination of Effects and Mechanisms of Action of Bacterial Amyloids on Antibiotic Resistance. In: Arluison, V., Wien, F., Marcoleta, A. (eds) Bacterial Amyloids. Methods in Molecular Biology, vol 2538. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2529-3_13

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  • DOI: https://doi.org/10.1007/978-1-0716-2529-3_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2528-6

  • Online ISBN: 978-1-0716-2529-3

  • eBook Packages: Springer Protocols

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