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Monitoring Molecular Assembly of Biofilms Using Quartz Crystal Microbalance with Dissipation (QCM-D)

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Bacterial Amyloids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2538))

Abstract

The structure and the functionality of biofilm proteins, the main components of the extracellular matrix, can be tuned by protein engineering. The use of binding kinetics data has been demonstrated in the characterization of recombinantly produced biofilm proteins to control their behavior on certain surfaces or under certain conditions. Quartz crystal microbalance with dissipation monitoring (QCM-D) allows measuring the change in resonance frequency and the energy loss and distribution upon the interaction of molecules with the surface. The characterization of the molecular assembly of curli biofilm proteins on different surfaces using QCM-D is presented here as a detailed protocol. The experimental procedure detailed in this chapter can be applied and modified for other biofilm proteins or subunits to determine their surface adsorption and kinetic binding characteristics.

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Author information

Authors and Affiliations

  1. Molecular Biology and Genetics Department, Yildiz Technical University, İstanbul, Turkey

    Esra Yuca

  2. UNAM-Institute of Materials Science and Nanotechnology, Bilkent University, Ankara, Turkey

    Urartu Özgür Şafak Şeker

Authors
  1. Esra Yuca
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  2. Urartu Özgür Şafak Şeker
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Corresponding author

Correspondence to Urartu Özgür Şafak Şeker .

Editor information

Editors and Affiliations

  1. CEA Saclay, Lab Léon Brillouin UMR12 CEA/CNRS, Gif sur Yvette Cedex, France

    Véronique Arluison

  2. Synchrotron SOLEIL, Gif-sur-Yvette Cedex, France

    Frank Wien

  3. Departamento de Biología, University of Chile, Santiago, Chile

    Andrés Marcoleta

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Yuca, E., Şeker, U.Ö.Ş. (2022). Monitoring Molecular Assembly of Biofilms Using Quartz Crystal Microbalance with Dissipation (QCM-D). In: Arluison, V., Wien, F., Marcoleta, A. (eds) Bacterial Amyloids. Methods in Molecular Biology, vol 2538. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2529-3_3

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  • DOI: https://doi.org/10.1007/978-1-0716-2529-3_3

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2528-6

  • Online ISBN: 978-1-0716-2529-3

  • eBook Packages: Springer Protocols

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