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NMR Analysis of Mammalian Glycolipids

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Glycolipids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2613))

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Abstract

Mammalian glycolipids play a variety of roles, often coupled with interactions with endogenous and exogenous molecules. The interactions can induce intracellular signaling and are the means by which glycolipids express biological phenotypes. Insights into the structure–function relationships of glycolipids (both glycan and lipid moieties) provide the basis for gaining an understanding of the mechanisms at play, an important area for further study. Solution nuclear magnetic resonance (NMR) spectroscopy is a unique and powerful method employed to provide, at the atomic level, structural information on glycolipids and other biomolecules in solutions. This chapter briefly describes how we measure NMR spectra of glycolipids and the information gained from NMR spectral analysis.

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Acknowledgments

This work was supported in part by JSPS KAKENHI Grant Number 19H03362.

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Authors and Affiliations

  1. Division of Structural Biology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai, Japan

    Yoshiki Yamaguchi

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  1. Yoshiki Yamaguchi
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Correspondence to Yoshiki Yamaguchi .

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Editors and Affiliations

  1. Department of Chemistry, Graduate School of Science, Osaka University, Osaka, Japan

    Kazuya Kabayama

  2. Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai, Japan

    Jin-ichi Inokuchi

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Yamaguchi, Y. (2023). NMR Analysis of Mammalian Glycolipids. In: Kabayama, K., Inokuchi, Ji. (eds) Glycolipids. Methods in Molecular Biology, vol 2613. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2910-9_14

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  • DOI: https://doi.org/10.1007/978-1-0716-2910-9_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2909-3

  • Online ISBN: 978-1-0716-2910-9

  • eBook Packages: Springer Protocols

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