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A Practical Guideline to Engineering Nonribosomal Peptide Synthetases

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Non-Ribosomal Peptide Biosynthesis and Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2670))

Abstract

The bioengineering of nonribosomal peptide synthetases (NRPSs) is a rapidly developing field to access natural product derivatives and new-to-nature natural products like scaffolds with changed or improved properties. However, the rational (re-)design of these often gigantic assembly-line proteins is by no means trivial and needs in-depth insights into structural flexibility, inter-domain communication, and the role of proofreading by catalytic domains—so it is not surprising that most previous rational reprogramming efforts have been met with limited success. With this practical guide, the result of nearly one decade of NRPS engineering in the Bode lab, we provide valuable insights into the strategies we have developed during this time for the successful engineering and cloning of these fascinating molecular machines.

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Acknowledgments

The authors are grateful to all current and past members from the Bode lab being involved in optimizing the NRPS engineering rules and protocols. Work in the Bode lab was supported by an ERC Advanced Grant (835108) and the LOEWE TBG research center.

Author information

Author notes

  1. Nadya Abbood and Leonard Präve contributed equally with all other contributors.

Authors and Affiliations

  1. Max-Planck-Institute for Terrestrial Microbiology, Department of Natural Products in Organismic Interactions, Marburg, Germany

    Nadya Abbood, Leonard Präve, Kenan A. J. Bozhueyuek & Helge B. Bode

  2. Molecular Biotechnology, Department of Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany

    Nadya Abbood, Leonard Präve, Kenan A. J. Bozhueyuek & Helge B. Bode

  3. Myria Biosciences AG, Basel, Switzerland

    Kenan A. J. Bozhueyuek

  4. Philipps-Universität Marburg, Chemische Biologie, Marburg, Germany

    Helge B. Bode

  5. Senckenberg Gesellschaft für Naturforschung, Frankfurt, Germany

    Helge B. Bode

Authors
  1. Nadya Abbood
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  2. Leonard Präve
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  3. Kenan A. J. Bozhueyuek
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  4. Helge B. Bode
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Corresponding authors

Correspondence to Kenan A. J. Bozhueyuek or Helge B. Bode .

Editor information

Editors and Affiliations

  1. Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA, USA

    Michael Burkart

  2. Faculty of Pharmacy, Kindai University, Higashi-Osaka, Osaka, Japan

    Fumihiro Ishikawa

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Abbood, N., Präve, L., Bozhueyuek, K.A.J., Bode, H.B. (2023). A Practical Guideline to Engineering Nonribosomal Peptide Synthetases. In: Burkart, M., Ishikawa, F. (eds) Non-Ribosomal Peptide Biosynthesis and Engineering. Methods in Molecular Biology, vol 2670. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3214-7_11

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  • DOI: https://doi.org/10.1007/978-1-0716-3214-7_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3213-0

  • Online ISBN: 978-1-0716-3214-7

  • eBook Packages: Springer Protocols

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