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Different Methods for Culturing Biofilms In Vitro

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Biofilm Infections

Abstract

The field of biofilm microbiology, while by no means new, has been experiencing significant “growing pains” as more and more researchers become involved. One of the underlying reasons is the lack of standardized methods for culturing biofilm communities. Many times, the culturing format will be unique to the study in question, resulting in difficulties when other labs attempt to confirm results produced by another lab. Another issue has been the limited utility of different culturing methods for the specific research questions being asked. For example, culturing formats designed to be accessible to microscopy are not always suited for other types of analyses, such as harvesting biofilm biomass for biochemical measurements.

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Authors and Affiliations

  1. Department of Microbiology, University of Washington, Seattle, WA, USA

    S. Brook Peterson, Yasuhiko Irie, Bradley R. Borlee, Keiji Murakami, Joe J. Harrison & Kelly M. Colvin

  2. Department of Microbiology - Box 357242, School of Medicine, University of Washington, 1959 NE Pacific Street, Seattle, WA, 98195-7242, USA

    Matthew R. Parsek

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  1. S. Brook Peterson
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  2. Yasuhiko Irie
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  3. Bradley R. Borlee
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  4. Keiji Murakami
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  5. Joe J. Harrison
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  6. Kelly M. Colvin
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  7. Matthew R. Parsek
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Correspondence to Matthew R. Parsek .

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Editors and Affiliations

  1. Faculty of Health Sciences, Department of International Health, Immu, University of Copenhagen, Blegdamsvej 3B, Copenhagen Ø, DK-2200, Denmark

    Thomas Bjarnsholt

  2. Afsnit 9301, Department Clinical Microbiology, H:S Rigshospitalet, Juliane Maries Vej 22, Copenhagen Ø, DK-2100, Denmark

    Peter Østrup Jensen

  3. Afsnit 9301, Department for Clinical Microbiology, H:S Rigshospitalet, Juliane Maries Vej 22, Copenhagen Ø, DK-2100, Denmark

    Claus Moser

  4. Faculty of Health Sciences, Department of International Health, Immu, University of Copenhagen, Blegdamsveg 3B, Copenhagen Ø, DK-2100, Denmark

    Niels Høiby

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Peterson, S.B. et al. (2011). Different Methods for Culturing Biofilms In Vitro. In: Bjarnsholt, T., Jensen, P., Moser, C., Høiby, N. (eds) Biofilm Infections. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-6084-9_15

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