Abstract
Candida rugosa lipase was immobilized by covalent binding on controlled pore silica (CPS) using glutaraldehyde as cross-linking agent under aqueous and nonaqueous conditions. The immobilized C. rugosa was more active when the coupling procedure was performed in the presence of a nonpolar solvent, hexane. Similar optima pH (7.5–8.0) was found for both free and immobilized lipase. The optimum temperature for the immobilized lipase was about 10°C higher than that for the free lipase. The thermal stability of the CPS lipase was also greater than the original lipase preparation. Studies on the operational stability of CPS lipase revealed good potential for recycling under aqueous (olive-oil hydrolysis) and nonaqueous (butyl butyrate synthesis) conditions.
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References
Leuenberger, H. G. W. (1990), Pure Appl. Chem. 62, 753–768.
Zaks, A. and Klibanov, A. M. (1988), J Biol. Chem. 263, 3194–3201.
Castro, H. F. and Anderson, W. A. (1995), Qu’mica Nova. 18, 544–554.
Balcxão, V. M., Paiva, A. L., and Xavier Malcata, F. (1996), Enzyme Microb. Technol. 18, 392–416.
Faber, K. (1997). Biotransformation in Organic Chemistry: A Textbook, Springer-Verlag, Berlin.
Castro, H. F., Pereira, E. B., and Anderson, W. A. (1996), J Brazilian Chem. Soc. 7, 219–224.
Castro, H. F., Oliveira, P. C., and Pereira, E. B. (1997), Biotechnol. Lett. 9, 229–232.
Castro, H. F., Oliveira, P. C., and Soares, C. M. F. (1997), Bol. SBCTA. 17, 237–241.
Xavier Malcata, E., Reyes, H. R., Garcia, H. S., Hill C. G., Jr., and Amundson, C. H. (1990), J Am. Oil Chem. Soc. 67, 890–910.
Mattiasson, B. and Adlecrentz, P. (1991), Tibtech. 9, 394–398.
Reslow, M., Adlercreutz, P., and Mattiason, B. (1988), European J. Biochem. 172, 573–578.
Norin, M., Boutelje, J., Holmberg, E., and Hurt, K. (1988), Appl. Microbiol. Biotechnol. 28, 527–530.
Wehtje, E., Adlercrentz, P., and Mattiason, B. (1993), Biotechnol. Bioeng. 41, 171–178.
Ison, A. P., Macrae, A. R., Smith, C. G., and Basley, J. (1994), Biotechnol. Bioeng. 43, 122–130.
Zanin, G. M. and De Moraes, F. F. (1994), Appl. Biotechnol. 45-46, 627–640.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951), J Biol. Chem. 193, 263–275.
Baron, M., Florencio, J. A., Zanin, G. M., Ferreira, A. G., Ennes, R., and Fontana, J. D. (1996), Appl. Biotechnol. 57-58, 605–615.
Mustranta, A., Forssell, P., and Poutanen, K. (1993), Enzyme Microb. Technol. 15, 133–139.
Gillies, B., Yamazaki, H., and Almstrong, D. (1987), Biotechnol. Lett. 9, 709–714.
Carta, G., Gainer, J. L., and Benton, A. H. (1991), Biotechnol. Bioeng. 37, 1004–1009.
Bosley, J. A. and Clayton, J. C. (1994), Biotechnol. Bioeng. 43, 934–938.
Reetz, M. T., Zonta, A., and Simpel Kamp, J. (1996), Biotechnol. Bioeng. 49, 527–534.
Laane, C., Boeren, S., Hilhorst, R., and Veeger, C. (1986), in Biocatalysis in Organic Media, Laane, C., Tramper, J., and Lilly, M. D., eds., Elsevier, Amsterdam, pp. 65–84.
Fukunaga, K., Minamijima, N., Sugimura, Y., Zhang, Z., and Nakao, K. (1996), J Biotechnol. 52, 81–88.
Castro, H. F., Oliveira, P. C., Soares, C. M. F., and Zanin, G. M. (1999), J Am. Oil Chem. Soc. 76(1), in press.
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Soares, C.M.F., De Castro, H.F., De Moraes, F.F., Zanin, G.M. (1999). Characterization and Utilization of Candida rugosa Lipase Immobilized on Controlled Pore Silica. In: Davison, B.H., Finkelstein, M. (eds) Twentieth Symposium on Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1604-9_68
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DOI: https://doi.org/10.1007/978-1-4612-1604-9_68
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