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Purification and Properties of Mycoplasma-Like Organisms from Diseased Plants

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Mycoplasma Diseases of Crops

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Abstract

Organisms resembling Mycoplasma (Class Mollicutes), first implicated by Japanese workers as the etiological agents of some “yellows” group of plant diseases (6,10), have been shown to be associated with many diseases that inflict damage to a variety of crops of economic importance (11,12). Most of these pleomorphic microbes are non-helical and are commonly referred to as mycoplasma-1ike organism(s) (MLO) because they have not yet been characterized through cultivation in a cell-free medium. Mycoplasmal etiology of about 100 plant diseases still remains to be demonstrated because Koch’s postulates cannot be satisfied. However, a few plant pathogenic mycoplasmas have been cultured in vitro, Koch’s poltulates fulfilled, and placed in a new genus Spiroplasma because they produce helical and motile filaments (7,14,15). Rapid progress has since been made on immunological, microbiological, biochemical, biophysical, and ultrastructural characterization of these mycoplasmas, especially of S. citri -- the etiological agent of citrus stubborn disease (1,3, 5,13,29,30).

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References

  1. Bové J.M. and Saillard, C. 1979. Cell biology of spiroplasmas. pps. 83–153. In: The Mycoplasmas. Vol. 3. Plant and Insect Mycoplasmas. R.F. Whitcomb, J.G. Tully, eds. Academic Press, New York.

    Google Scholar 

  2. Castello, J.R. , Shiel, P., Austin, J.A., Jones, F., Craft, C. and Delgado, G.. 1984. Partial purification of ash yellows and elm yellows mycoplasma-1ike organisms from infected symptomatic periwinkle plants. Phytopathology 74: 804, Abst.

    Google Scholar 

  3. Chen, T.A. and Davis, R.E. 1979. Cultivation of spiroplasmas. pps. 65–82. In: The Mycoplasmas. Vol. 3. Plant and Insect Mycoplasmas. R.F. WhitcombJ.G. Tully, eds. Academic Press, New York.

    Google Scholar 

  4. Clark, M.F., Barbara, D.J. and Davis, D.L. 1983. Production and characteristics of antisera to Spiroplasma citri and clover phyllody—associated antigens derived from plants. Ann. Appl. Biol. 103: 251–259.

    Article  Google Scholar 

  5. Daniels, M.J. 1979. Mechanisms of spiroplasma pathogenicity. Pages 209–277. In: The Mycoplasmas. Vol. 3. Plant and Insect Mycoplasmas. R.F. Whitcomb, J.G. Tully, eds. Academic Press, New York.

    Google Scholar 

  6. Doi, Y., Terananka, M., Yora, K. and Asuyama, H. 1967. Mycoplasma- or PLT—like microorganisms found in the phloem elements of plants infected with mulberry dwarf, potato witches’ broom, aster yellows or Paulownia witches’ broom. Ann. Phytopathol. Soc. Japan 33: 259–266.

    Google Scholar 

  7. Fudl-Allah, A., Calavan, E.C. and Igwegbe, E.C.K. 1972. Culture of a mycoplasma–1ike organism associated with stubborn disease of citrus. Phytopathology 62: 729–731.

    Article  Google Scholar 

  8. Haggis, G.H. and Sinha, R.C. 1978. Scanning electron microscopy of mycoplasma-1ike organisms after freeze fracture of plant tissues affected with clover phyllody and aster yellows. Phytopathology 68: 677–680.

    Article  Google Scholar 

  9. Hirumi, H. and Maramorosch, K. 1973. Mycoplasma and mycoplasma-1ike agents of human, animal and plant diseases. Ann. N.Y. Acad. Sei. 225: 201–222.

    Article  Google Scholar 

  10. Ishiie, T., Doi, Y., Yora, K. and Asuyama, H. 1967. Suppressive effects of antibiotics of tetracycline group on symptom development of mulberry dwarf disease. Ann. Phytopathol. Soc. Japan 33: 267–275.

    CAS  Google Scholar 

  11. Maramorosch, K., Granados, R.R. and Hirumi, H. 1970. Mycoplasma disease of plants and insects. Adv. Virus. Res. 16: 135–193.

    Article  PubMed  CAS  Google Scholar 

  12. McCoy, R.E. 1979. Mycoplasmas and yellows diseases. Pages 229–259. In: The Mycoplasmas. Vol. 3. Plant and Insect Mycoplasmas. R.F. Whitcomb, J.G. Tully, eds. Academic Press, New York.

    Google Scholar 

  13. Padhi, S.B., Mcintosh, A.H. and Maramorosch. K. 1977. Characterization and identification of spiroplasmas by Polyacrylamide gel electrophoresis. Phytopath Z 90: 268–272.

    Article  Google Scholar 

  14. Saglio, P., L’Hospital, M., Laflèche, D., Dupont, G., Bové J.M., Tully, G. and Freundt, E.A. 1973. Spiroplasma citri gen. and sp. n.: a mycoplasma-1ike organism associated with “stubborn” disease of citrus. Int. J. Syst. Bacteriol. 23: 191–204.

    Article  CAS  Google Scholar 

  15. Saglio, P., Laflèuche, D., Bonissol, C. and Bové J.M. 1971. Isolement, culture et observation au microscope electronique des structure de type mycoplasma associees a la maladie du Stubborn des agrumes et leur comparaison avec les structures observees dans le cas de la maladie du Greening des agrumes. Physiol. Veg. 9: 569–582.

    Google Scholar 

  16. Sinha, R.C. 1974. Purification of mycoplasma-like organisms from China aster plants affected with clover phyllody. Phytopathology 64: 1156–1158.

    Article  Google Scholar 

  17. Sinha, R.C. 1976. Ultrastructure of mycoplasma-like organisms purified from clover phyl1ody-affected plants. J. Ultrastruct. Res. 54: 183–189.

    Article  PubMed  CAS  Google Scholar 

  18. Sinha, R.C. 1979. Lipid composition of mycoplasma-1ike organisms purified from clover phyllody and aster yellows-affected plants. Phytopath. Z. 96: 132–139.

    Article  CAS  Google Scholar 

  19. Sinha, R.C. 1979. Purification and serology of mycoplasma-1ike organisms from aster yellows-infected plants. Can. J. Plant Pathol. 1: 65–70.

    Article  Google Scholar 

  20. Sinha, R.C. 1983. Physical techniques for purification of mycoplasmas from plant tissues, pps. 243–247. In: Methods in Mycoplasmology. Vol. 2. Diagnostic mycoplasmology. J.G. Tully, S. Razin, eds. Academic Press, New York.

    Google Scholar 

  21. Sinha, R.C. 1983. Relative concentration of mycoplasma-1ike organisms in plants at various times after infection with aster yellows. Can. J. Plant Pathol. 5: 7–10.

    Article  Google Scholar 

  22. Sinha, R.C. 1983. The aster yellows controversy. Current status. The Yale J. Biol. Med. 56: 737–743.

    CAS  Google Scholar 

  23. Sinha, R.C. 1984. Transmission mechanisms of mycoplasma-1ike organisms by leafhopper vectors. pps. 93–109. In: Current Topics in Vector Research, Vol. 2. K.F. Harris, ed. Praeger Scientific, New York.

    Google Scholar 

  24. Sinha, R.C. and Benhamou, N. 1983. Detection of mycoplasma-1ike organism antigens from aster yellow—diseased plants by two serological methods. Phytopathology 73: 1 199–1202.

    Google Scholar 

  25. Sinha, R.C. and Chiykowski, L.N. 1984. Purification and serological detection of mycoplasma-1ike organisms from plants affected by peach eastern X-disease. Can. J. Plant Pathol. 6: 200–205.

    Article  Google Scholar 

  26. Sinha, R.C. and Madhosingh, C. 1980. Proteins of mycoplasma-1ike organisms purified from clover phyllody and aster yellows—affected plants. Phytopath. Z. 99: 294–300.

    Article  CAS  Google Scholar 

  27. Sinha, R.C. and Paliwal, Y.C. 1969. Association, development and growth cycle of mycoplasma-1ike organisms in plants affected with clover phyllody. Virology 39: 759–767.

    Article  PubMed  CAS  Google Scholar 

  28. Tully, J.G. 1973. Biological and serological characteristics of the acholeplasmas. Ann. N.Y. Acad. Sei. 225: 74–93.

    Article  Google Scholar 

  29. Williamson, D.L. 1982. Serological characterization of spiroplasmas and other mycoplasmas, pps. 240–267. In: Plant and Insect Mycoplasma Techniques. M.J. Daniels, P.G. Markhan, eds. John Wiley and Sons, New York.

    Google Scholar 

  30. Williamson, D.L. and Whitcomb, R.F. 1983. Special serological tests for spiroplasma identification. pps. 249–259. In: Methods in Mycoplasmology. Vol. 2. Diagnostic mycoplasmology. J.G. Tully, S. Razin, eds., Academic Press, New York.

    Google Scholar 

  31. Wolanski, B. and Maramorosch, K. 1970. Negatively stained mycoplasmas: fact or artifact. Virology 42: 319–327.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Chemistry and Biology Research Institute Agriculture Canada, Ottawa, Ontario, K1A OC6, Canada

    R. C. Sinha

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Editors and Affiliations

  1. Department of Entomology and Economic Zoology, Cook College Rutgers—The State University, New Brunswick, 08903, New Jersey, USA

    Karl Maramorosch

  2. IUFRO Working Party on Mycolplasma Diseases, A-61 Alkananda Kalkaji, 110019, New Delhi, India

    S. P. Raychaudhuri

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© 1988 Springer-Verlag New York Inc.

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Sinha, R.C. (1988). Purification and Properties of Mycoplasma-Like Organisms from Diseased Plants. In: Maramorosch, K., Raychaudhuri, S.P. (eds) Mycoplasma Diseases of Crops. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3808-9_2

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  • DOI: https://doi.org/10.1007/978-1-4612-3808-9_2

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-8360-7

  • Online ISBN: 978-1-4612-3808-9

  • eBook Packages: Springer Book Archive

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