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Multiplication of Meristematic Tissue: A New Tissue Culture System for Radiata Pine

  • Chapter
Genetic Manipulation of Woody Plants

Part of the book series: Basic Life Sciences ((BLSC,volume 44))

Abstract

A subculturable meristematic tissue system capable of plantlet regeneration has been developed for Pinus radiata. Multiplication was achieved by the continuous production of meristematic tissue on a modified Lepoivre medium containing 5 mg/1 BAP (LP5). This tissue has been maintained for 2.5 years to date. Meristematic nodules multiplied for one year consisted of three zones: the outer meristematic layer, a bulky layer of vacuolated cells, and friable cells containing tannins and degrading cell walls. Cavities (hollows) were often present near the center of meristematic nodules. Natural separation of meristematic nodules contributed to the multiplication process. Factors affecting the success of the system were studied because there was a large variation in response. Embryos formed shoots and multipliable meristematic tissue in similar percentages. Seedlot was not a major factor in multiplication of meristematic tissue. Half-strength LP5 medium proved best for the first 12 weeks in culture, whereas LP5 medium proved best after six months in culture. Both gelrite and liquid induced vitrification, and habituation on LP5 medium had not occurred after 20 months in culture. One of the best embryos produced 5,480 pieces of meristematic tissue in 13.5 months. In a separate experiment with the same clone, an average of 68.4 shoots elongated from each piece of tissue. It was estimated that 260,000 trees could be produced from a single, good reacting seed in 2.5 years. Genetic stability and automation, two prerequisites for commercial use, are also discussed as well as the potential for gene transfer.

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Author information

Authors and Affiliations

  1. Ministry of Forestry, Forest Research Institute, Rotorua, New Zealand

    Jenny Aitken-Christie, Adya P. Singh & Helen Davies

Authors
  1. Jenny Aitken-Christie
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  2. Adya P. Singh
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  3. Helen Davies
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Editor information

Editors and Affiliations

  1. Michigan State University, East Lansing, Michigan, USA

    James W. Hanover  & Daniel E. Keathley  & 

  2. Council for Research Planning in Biological Sciences, Inc., Washington, D.C., USA

    Claire M. Wilson  & Gregory Kuny  & 

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© 1988 Plenum Press, New York

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Aitken-Christie, J., Singh, A.P., Davies, H. (1988). Multiplication of Meristematic Tissue: A New Tissue Culture System for Radiata Pine. In: Hanover, J.W., Keathley, D.E., Wilson, C.M., Kuny, G. (eds) Genetic Manipulation of Woody Plants. Basic Life Sciences, vol 44. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1661-9_26

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  • DOI: https://doi.org/10.1007/978-1-4613-1661-9_26

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8922-7

  • Online ISBN: 978-1-4613-1661-9

  • eBook Packages: Springer Book Archive

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