Abstract
We have recently reported1–3 on a new methodology for amino acid analysis (AAA) that relies on the reaction of free amino acids with Edman’s reagent, phenylisothiocyanate (PITC), to form the phenylthiocarbamyl (PTC) derivatives, which are subsequently separated and quantitated using a reverse phase gradient liquid chromatography (LC) system for analysis. This work extended the methodology for AAA using PITC introduced several years ago by Tarr and co-workers4–5, and appeared concurrently with a report by Heinrikson and Meredith6 describing similar methodology for AAA. The method was shown to be reliable, accurate, and extremely rapid (12 minute) with a maximum one picomole detection limit for each amino acid. This new method provides improved capabilities in comparison with conventional ion-exchange, post-column ninhydrin reaction systems. In addition, linear response in the 1020131000 pmol range was demonstrated as well as a high degree of reproducibility1. This system has been recently commercialized under the name PICO·TAG™ Amino Acid Analysis System3.
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References
B. A. Bidlingmeyer, S. A. Cohen and T. L. Tarvin, paper presented at the International Symposium on HPLC in the Biological Sciences Conference in Melbourne, Australia, February 20–22, 1984.
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© 1987 Plenum Press, New York
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Cohen, S.A., Tarvin, T.L., Bidlingmeyer, B.A. (1987). Amino Acid Analysis Using Pre-Column Derivatization with Phenylisothiocyanate: Matrix Effects and Tryptophan Analysis. In: L’Italien, J.J. (eds) Proteins. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1787-6_22
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DOI: https://doi.org/10.1007/978-1-4613-1787-6_22
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