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Conjugal Plasmid Transfer in Bacillus Thuringiensis

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Plasmids in Bacteria

Abstract

Bacillus thuringiensis (BT) is a gram-positive, sporeforming bacterium distinguished by its ability to produce parasporal protein crystals. These proteins, collectively known as δ-endotoxins, are toxic to a number of lepidopteran insects of economic importance as well as to certain dipterans which are important vectors of human disease (7). Over 20 different varieties of B. thuringiensis are currently recognized on the basis of flagellar antigens (6) and immunological (19) and toxicity (8) properties of the parasporal crystals.

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References

  1. Anagnostopoulos, C., and J. Spizizen (1961) Requirements for transformation in Bacillus subtilis. J. Bact. 81: 741–746.

    PubMed  CAS  Google Scholar 

  2. Brefort, G., M. Magot, H. Ionesco, and M. Sebald (1977) Characterization and transferability of Clostridium perfringens plasmids. Plasmid 1: 52–66.

    Article  PubMed  CAS  Google Scholar 

  3. Carlton, B.C., and B.J. Brown (1981) Gene mutation. In Manual of Methods for General Bacteriology, P. Gerhardt, ed. Am. Soc. Microbiol., Washington, D.C.

    Google Scholar 

  4. Clark, A.J., and G.J. Warren (1979) Conjugal transmission of plasmids. An. Rev. Genet. 13: 99–125.

    Article  CAS  Google Scholar 

  5. Cullum, J., J.F. Collins, and P. Broda (1978) Factors affecting the kinetics of progeny formation with F’lac in Escherichia coli K12. Plasmid 1: 536–544.

    Article  PubMed  CAS  Google Scholar 

  6. de Barjac, H. (1981) Identification of H–serotypes of Bacillus thuringiensis. In Microbial Control of Pests and Plant Diseases, 1970–1980, H.D. Burges, ed. Academic Press, New York, pp. 35–43.

    Google Scholar 

  7. Dulmage, H.T. (1979) Genetic manipulation of pathogens: Selection of different strains. In Genetics in Relation to Insect Management, M.A. Hoy and J.J. McKelvey, Jr., eds. Rockefeller Foundation, New York, pp. 116–127.

    Google Scholar 

  8. Dulmage, H.T. and cooperators. (1981) Insecticidal activity of isolates of Bacillus thuringiensis and their potential for pest control. In Microbial Control of Pests and Plant Diseases, 1970–1980, H.D. Burges, ed. Academic Press, New York, pp. 193–222.

    Google Scholar 

  9. Dunny, G.M., B.L. Brown, and D.B. Clewell (1978) Induced cell aggregation and mating in Streptococcus faecalis: Evidence for a bacterial sex pheromone. Proc. Natl. Acad. Sci., USA 75: 3479–3483.

    Article  PubMed  CAS  Google Scholar 

  10. Dunny, G.M., R.A. Craig, R.L. Carron, and D.B. Clewell (1979) Plasmid transfer in Streptococcus faecalis: Production of multiple sex pheromones by recipients. Plasmid 2:454–465.

    Article  PubMed  CAS  Google Scholar 

  11. González, Jr., J.M., and B.C. Carlton (1980) Patterns of plasmid DNA in crystalliferous and acrystalliferous strains of Bacillus thuringiensis. Plasmid 3: 92–98.

    Article  PubMed  Google Scholar 

  12. González, Jr., J.M., H.T. Dulmage, and B.C. Carlton (1981) Correlation between specific plasmids and 6-endotoxin production in Bacillus thuringiensis. Plasmid 5: 351–365.

    Article  Google Scholar 

  13. González, Jr., J.M., and B.C. Carlton (1982) Plasmid transfer in Bacillus thuringiensis. In Genetic Exchange: A Celebration and a New Generation, U.N. Streips, S.H. Goodgal, W.R. Guild, and G.A. Wilson, eds. Dekker, New York. pp. 85–95.

    Google Scholar 

  14. González, Jr., J.M., B.J. Brown, and B.C. Carlton (1982) Transfer of Bacillus thuringiensis plasmids coding for δ-endotoxin among strains of B. thuringiensis and B. cereus. Proc. Natl. Acad. Sci., USA 79: 6951–6955.

    Article  PubMed  Google Scholar 

  15. González, Jr., J.M., and B.C. Carlton (1984) A large transmissible plasmid is required for crystal toxin production in Bacillus thuringiensis variety israelensis. Plasmid 11: 28–38.

    Article  PubMed  Google Scholar 

  16. Hopwood, D.A., and M.J. Merrick (1977) Genetics of antibiotic production. Bacteriol. Rev. 41: 595–635.

    PubMed  CAS  Google Scholar 

  17. Jacob, A.E., and S.J. Hobbs (1974) Conjugal transfer of plasmid–borne multiple antibiotic resistance in Streptococcus faecalis var. zymogenes. J. Bact. 117: 360–372.

    PubMed  CAS  Google Scholar 

  18. Klier, A., C. Bourgouin, and G. Rapoport (1983) Mating between Bacillus subtilis and Bacillus thuringiensis and transfer of cloned crystal genes. Mol. Gen. Genet. 191: 257–262.

    Article  CAS  Google Scholar 

  19. Krywienczyk, J., and T.A. Angus (1967) A serological comparison of several crystalliferous insect pathogens. J. Invert. Pathol. 9: 126–128.

    Article  Google Scholar 

  20. Orrego, C., M. Arnaud, and H.O. Halvorson (1978) Bacillus subtilis 168. Genetic transformation mediated by outgrowing spores: Necessity for cell contact. J. Bact. 134: 973–981.

    PubMed  CAS  Google Scholar 

  21. Stewart, G.J., C.A. Carlson, and J.L. Ingraham (1983) Evidence for an active role of donor cells in natural transformation of Pseudomonas stutzeri. J. Bact. 156: 30–35.

    PubMed  CAS  Google Scholar 

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© 1985 Plenum Press, New York

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Chapman, J.S., Carlton, B.C. (1985). Conjugal Plasmid Transfer in Bacillus Thuringiensis . In: Helinski, D.R., Cohen, S.N., Clewell, D.B., Jackson, D.A., Hollaender, A. (eds) Plasmids in Bacteria. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2447-8_33

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  • DOI: https://doi.org/10.1007/978-1-4613-2447-8_33

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9487-0

  • Online ISBN: 978-1-4613-2447-8

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