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High-Sensitivity Differential Scanning Calorimetry in the Study of Biomembranes and Related Model Systems

  • Chapter
Methods in Membrane Biology

Abstract

Differential scanning calorimetry (DSC) currently finds wide application in studies of the thermotropic behavior of lipids in biological membranes and related systems (Chapman, 1975a,b; Scheidler and Steim, 1975). Almost without exception these studies have employed calorimetric equipment which is limited to very small sample sizes and relatively high scan rates, so that only low sensitivity is realized with the aqueous solutions and suspensions which are of interest in biology. The recent development of scanning calorimeters especially adapted for applications involving relatively dilute solutions and suspensions (Gill and Beck, 1965; Tsong et al., 1970; Jackson and Brandts, 1970; Ross and Goldberg, 1974; Grubert and Ackermann, 1974; Privalov et al., 1975; and Suurkuusk et al., 1976) opens up the possibility of more searching and quantitative studies of these systems. The present chapter outlines the fundamental principles of DSC, and discusses several recent applications of high-sensitivity DSC to membranes and related systems, with particular reference to the most highly developed scanning calorimeter currently available, the one designed by Privalov (Privalov et al., 1975).

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  1. Department of Chemistry, Yale University, New Haven, Connecticut, USA

    Susan Mabrey & Julian M. Sturtevant

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  1. Susan Mabrey
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  1. Laboratory of Cell Biology, National Heart and Lung Institute, Bethesda, Maryland, USA

    Edward D. Korn

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Mabrey, S., Sturtevant, J.M. (1978). High-Sensitivity Differential Scanning Calorimetry in the Study of Biomembranes and Related Model Systems. In: Korn, E.D. (eds) Methods in Membrane Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-4036-2_3

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  • DOI: https://doi.org/10.1007/978-1-4613-4036-2_3

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