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Structure and Dynamics of the Liver Microsomal Monoxygenase System

  • Chapter
Fluorescence Studies on Biological Membranes

Part of the book series: Subcellular Biochemistry ((SCBI,volume 13))

  • 152 Accesses

Abstract

Cell membranes regulate a variety of cellular processes ranging from permeability, transport, and excitability to intercellular interaction, morphological differentiation, and fusion. Numerous models have been advanced to characterize the organization of lipids and proteins in cell membranes. Today, there is substantial agreement on the “fluid mosaic” model (Singer and Nicholson, 1972), which emphasizes the dynamic behavior of the membrane components. Both lipids and proteins can undergo a variety of motions: rotational motion around the axis perpendicular to the plane of the membrane; lateral diffusion in the plane of the membrane; in addition, lipids can “flip-flop” (exchange from one monolayer to the other) and undergo trans-gauche conformational changes in the phospholipid acyl chains, which give rise to an increased segmental mobility toward the center of the bilayer. Since the fluid mosaic model has been proposed, its rather crude and generalizing picture has been filled with some details. The refined picture shows a dynamic membrane in which molecular associations are tightly controlled, and in which long-range lateral motions are surprisingly restricted.

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Abbreviations

ANS:

1-anilino-naphthlene sulfonate

ANM:

N-(1-anilinonaph-4yl)maleimide

DPH:

1,6-diphenyl-1,3,5-hexatriene

DMPC:

dimyristoylphosphatidylcholine

DOPC:

dioleylphosphatidylcholine

DPPC:

dipalmitoyphosphatidylcholine

E:

eosin

EPR:

electronparamagnetic

FITC:

fluorescein isothiocyanate

FRAP:

fluorescence recovery after photobleaching

I-a, I-b, I-c, I-d:

P-450 isozymes isolated from rat liver

LM-2, LM-4:

P-450 isozymesisolated from rabbit liver

NMR:

nuclear magnetic resonance

P-450:

microsomal cytochrome P-450

P-450b :

P-450 isozyme isolated from rat liver

PA:

dipalmitoylphosphatidic acid

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PS:

phosphatidylserine

PUFA:

polyunsaturated fatty acid

SDS-PAGE:

polyacrylamide gel electrophoresis

ST-EPR:

saturation transfer EPR

Tc :

temperature of the gel to liquid-crystalline phase transition

TRSP:

time-resolved spatial photometry

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  1. Josef Gut

    Present address: Stanford University, Stanford, California, 94025, USA

  2. Barbara C. Kunz

    Present address: National Institutes of Health, Bethesda, Maryland, 20205, USA

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  1. Laboratory of Biochemistry, Swiss Federal Institute of Technology, CH-8092, Zürich, Switzerland

    Christoph Richter, Josef Gut & Barbara C. Kunz

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  1. University of Antwerp, Antwerp, Belgium

    H. J. Hilderson

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Richter, C., Gut, J., Kunz, B.C. (1988). Structure and Dynamics of the Liver Microsomal Monoxygenase System. In: Hilderson, H.J. (eds) Fluorescence Studies on Biological Membranes. Subcellular Biochemistry, vol 13. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-9359-7_8

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  • DOI: https://doi.org/10.1007/978-1-4613-9359-7_8

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-9361-0

  • Online ISBN: 978-1-4613-9359-7

  • eBook Packages: Springer Book Archive

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