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Methods of Cryopreservation in Fungi

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Laboratory Protocols in Fungal Biology

Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

Traditional method of the routine subculturing by transfer of fungal cultures from staled to fresh media is not a very practical means of storing large numbers of fungal cultures. It is time-consuming, prone to contamination, and does not prevent genetic and physiological changes. At present, besides freeze-drying (lyophilization), cryopreservation seems to be the best preservation technique available for fungi.

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Notes

  1. 1.

    Homolka, unpublished results.

  2. 2.

    All chemicals and devices named can be replaced with other ones produced by other renowned companies.

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Authors and Affiliations

  1. Department of Ecology of Microorganisms, Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4, 142 20, Czech Republic

    Ladislav Homolka

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  1. Ladislav Homolka
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Correspondence to Ladislav Homolka .

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Editors and Affiliations

  1. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Vijai Kumar Gupta

  2. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Maria G. Tuohy

  3. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Manimaran Ayyachamy

  4. , Manufacturing Sciences and Technology, Pfizer Ireland Pharmaceuticals, The Pfizer Biotech Campus at Grange Castle, Clondalkin, N/A, Dublin, Ireland

    Kevin M. Turner

  5. , School of Natural Sciences, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Anthonia O’Donovan

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Homolka, L. (2013). Methods of Cryopreservation in Fungi. In: Gupta, V., Tuohy, M., Ayyachamy, M., Turner, K., O’Donovan, A. (eds) Laboratory Protocols in Fungal Biology. Fungal Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-2356-0_2

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