Skip to main content

Bioprocess Engineering Aspects of Biodiesel and Bioethanol Production from Microalgae

  • Chapter
  • First Online:
Advanced Biofuels and Bioproducts

Abstract

Rapid increase of atmospheric carbon dioxide together with depleted supplies of fossil fuel has led to an increased commercial interest in renewable fuels. Due to their high biomass productivity, rapid lipid accumulation and high carbohydrate storage capacity, microalgae are viewed as promising feedstocks for carbon-neutral biofuels. This chapter discusses process engineering steps for the production of biodiesel and bioethanol from microalgal biomass (harvesting, dewatering, pre-treatment, lipid extraction, lipid transmethylation, anaerobic fermentation). The suitability of microalgal lipid compositions for biodiesel conversion and the feasibility of using microalgae as raw materials for bioethanol production will also be evaluated. Specific to biodiesel production, the chapter provides an updated discussion on two of the most commonly used technologies for microalgal lipid extraction (organic solvent extraction and supercritical fluid extraction) and evaluates the effects of biomass pre-treatment on lipid extraction kinetics.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Andrich G, Nesti U, Venturi F, Zinnai A, Fiorentini R (2005) Supercritical fluid extraction of bioactive lipids from the microalgae Nannochloropsis sp. Eur J Lipid Sci Technol 107:381–386

    Article  CAS  Google Scholar 

  2. Andrich G, Zinnai A, Nesti U, Venturi F, Fiorentini R (2006) Supercritical fluid extraction of oil from microalga Spirulina (Arthrospira) platensis. Acta Aliment 35(2):195–203

    Article  CAS  Google Scholar 

  3. Applied separations website (2009) http://www.appliedseparations.com/Supercritical/Lab_Inst/Analytical/SFE_2.asp. Accessed 30 June 2009

  4. Ballesteros I, Ballesteros M, Manzanares P, Negro MJ, Oliva JM, Sailez F (2008) Dilute sulfuric acid pretreatment of cardoon for ethanol production. Biochem Eng J 42:84–91

    Article  CAS  Google Scholar 

  5. Becker EW (1994) Microalgae: biotechnology and microbiology. In: Baddiley J, Carey NH, Higgins IJ, Potter WG (eds) Cambridge studies in biotechnology. Cambridge University Press, New York, p 178

    Google Scholar 

  6. Brown MR, Jeffrey SW, Volkman JK, Dunstan GA (1997) Nutritional properties of microalgae for mariculture. Aquaculture 151:315–331

    Article  CAS  Google Scholar 

  7. Canela APRF, Rosa PTV, Marques MOM, Meireles MAA (2002) Supercritical fluid extraction of fatty acids and carotenoids from the microalgae Spirulina maxima. Ind Eng Chem Res 41:3012–3018

    Article  CAS  Google Scholar 

  8. Casey GP, Ingledew WM (1986) Ethanol tolerance in yeasts. Crit rev microbiol 13:219–280

    Article  CAS  Google Scholar 

  9. Chen M, Zhao J, Xia L (2009) Comparison of four different chemical pretreatments of corn stover for enhancing enzymatic digestibility. Biomass Bioenerg 33:1381–1385

    Article  CAS  Google Scholar 

  10. Cheung P (1999) Temperature and pressure effects on supercritical carbon dioxide extraction of n-3 fatty acids from red seaweed. Food Chem 65:399–403

    Article  CAS  Google Scholar 

  11. Chisti Y (2007) Research review paper: biodiesel from microalgae. Biotechnol Adv 25:294–306

    Article  CAS  Google Scholar 

  12. Chisti Y, Moo-Young M (1986) Review: disruption of microbial cells for intracellular products. Enzyme Microb Technol 8:194–204

    Article  CAS  Google Scholar 

  13. Choi S, Nguyen MT, Sim SJ (2010) Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production. Bioresour Technol 101:5330–5336

    Article  CAS  Google Scholar 

  14. Christie WW (2007) Methylation of fatty acids—a beginner’s guide. http://www.lipidlibrary.co.uk/topics/methests/index.htm. Accessed 5 Apr 2009

  15. Danquah MK, Gladman B, Moheimani N, Forde GM (2009) Microalgal growth characteristics and subsequent influence on dewatering efficiency. Chem Eng J 151:73–78

    Article  CAS  Google Scholar 

  16. De Angelis L, Rise P, Giavarini F, Galli C, Bolis CL, Colombo ML (2005) Marine macroalgae analyzed by mass spectrometry are rich sources of polyunsaturated fatty acids. J Mass Spectrom 40:1605–1608

    Article  Google Scholar 

  17. Demirbas A (2008) Comparison of transesterification methods for production of biodiesel from vegetable oils and fats. Energ Convers Manage 49:125–130

    Article  CAS  Google Scholar 

  18. Demirbas A (2009) Biofuels from agricultural biomass. Energ Source Part A, 31:1573–1582

    Google Scholar 

  19. Demirbas A, Karslioglu S (2007) Biodiesel production facilities from vegetable oils and animal fats. Energy Sources Part A 29:133–141

    Article  CAS  Google Scholar 

  20. Dunstan GA, Volkman JK, Barrett SM, Garland CD (1993) Changes in the lipid composition and maximisation of the polyunsaturated fatty acid content of three microalgae grown in mass culture. J appl phycol 5:71–83

    Article  CAS  Google Scholar 

  21. Dunstan GA, Volkman JK, Jeffrey SW, Barrett SM (1992) Biochemical composition of microalgae from the green algal classes Chlorophyceae and Prasinophyceae. 2. Lipid classes and fatty acids. J Exp Mar Biol Ecol 161:115–134

    Article  CAS  Google Scholar 

  22. Fajardo AR, Cerdan LE, Medina AR, Fernandez FGA, Moreno PAG, Grima EM (2007) Lipid extraction from the microalga Phaedactylum tricornutum. Eur J Lipid Sci Tech 109:120–126

    Article  CAS  Google Scholar 

  23. Folch J, Ascoli I, Lees M, Meath JA, Lebaron FN (1951) Preparation of lipide extracts from brain tissue. J Biol Chem 191(2):833–841

    CAS  Google Scholar 

  24. Galbe M, Zacchi G (2007) Pretreatment of lignocellulosic materials for efficient bioethanol production. Adv Biochem Eng Biotechnol 108:41–65

    CAS  Google Scholar 

  25. Garcia Sanchez R, Karhumaa K, Fonseca C (2010) Improved xylose and arabinose utilization by an industrial recombinant Saccharomyces cerevisiae strain using evolutionary engineering. Biotechnol Biofuels 3:13

    Article  Google Scholar 

  26. Guckert JB, Cooksey KE, Jackson LL (1988) Lipid solvent systems are not equivalent for analysis of lipid classes in the microeukaryotic green alga, Chlorella. J Microbiol Methods 8:139–149

    Article  CAS  Google Scholar 

  27. Harun R, Singh M, Forde GM (2010) Bioprocess engineering of microalgae to produce a variety of consumer products. Renew Sustain Energy Rev 14:1037–1047

    Article  CAS  Google Scholar 

  28. Herrero M, Cifuentes A, Ibanez E (2006) Sub- and supercritical fluid extraction of functional ingredients from different natural sources: plants, food-by-products, algae and microalgae, a review. Food Chem 98:136–148

    Article  CAS  Google Scholar 

  29. Hu G, Heitmann JA, Rojas OJ (2008) Feedstock pre-treatment strategies for ethanol from wood, bark and forest residues. Bioresour Technol 3:270–294

    Google Scholar 

  30. Kates M (1986) Definition and classification of lipids. In: Bordon RH, Knippenberg PH (eds) Techniques of lipidology: isolation, analysis, and identification of lipids. Elsevier, Amsterdam

    Google Scholar 

  31. Lang X, Dalai AK, Bakhshi NN, Reaney MJ, Hertz PB (2001) Preparation and characterization of bio-diesels from various bio-oils. Bioresour Technol 80:53–62

    Article  CAS  Google Scholar 

  32. Lee JY, Yoo C, Jun SY, Ahn CY, Oh HM (2010) Comparison of several methods for effective lipid extraction from microalgae. Bioresour Technol 101:S75–S77

    Article  CAS  Google Scholar 

  33. Lee SJ, Yoon BD, Oh HM (1998) Rapid method for the determination of lipid from the green algae Botryococcus braunii. Biotechnol Tech 7:553–556

    Article  Google Scholar 

  34. Lin Y, Tanaka S (2006) Ethanol fermentation from biomass resources: current state and prospects. Appl Microbiol Biotechnol 69:627–642

    Article  CAS  Google Scholar 

  35. Luque de Castro MD, Garcia-Ayuso LE (1998) Soxhlet extraction of solid materials: an outdated technique with a promising innovative future. Anal Chim Acta 369:1–10

    Article  CAS  Google Scholar 

  36. Lynd LR (1996) Overview and evaluation of fuel ethanol from cellulosic biomass: technology, economics, the environment, and policy. Annu Rev Energ Environ 21:403–465

    Article  Google Scholar 

  37. Macias-Sanchez MD, Mantell C, Rodriguez M, de la Ossa EM, Lubian LM, Montero O (2007) Supercritical fluid extraction of carotenoids and chlorophyll a from Synechococcus sp. J Supercrit Fluid 39:323–329

    Article  CAS  Google Scholar 

  38. Madigan MT, Martinko JM, Parker J (2000) Nutrition and metabolism. In: Madigan MT, Martinko JM, Parker J (eds) Brock biology of microbiology. Prentice-Hall, New Jersey

    Google Scholar 

  39. Medina AR, Grima EM, Gimenez AG, Ibanez MJ (1998) Downstream processing of algal polyunsaturated fatty acids. Biotechnol Adv 16(3):517–580

    Article  CAS  Google Scholar 

  40. Mendes RL, Coelho JP, Fernandes HL, Marrucho IJ, Cabral JM, Novais JM, Palavra AF (1995) Applications of supercritical CO2 extraction to microalgae and plants. J Chem Technol Biotechnol 62:53–59

    Article  CAS  Google Scholar 

  41. Mendes RL, Nobre BP, Cardoso MT, Pereira AP, Palavra AF (2003) Supercritical carbon dioxide extraction of compounds with pharmaceutical importance from microalgae. Inorg Chim Acta 357:328–334

    Article  Google Scholar 

  42. Mendes RL, Reis AD, Palavra AF (2006) Supercritical CO2 extraction of gamma-linolenic acid and other lipids from Arthrospira (Spirulina) maxima: comparison with organic solvent extraction. Food Chem 99:57–63

    Article  CAS  Google Scholar 

  43. Mendes-Pinto MM, Raposo MFJ, Bowen J, Young AJ, Morais R (2001) Evaluation of different cell disruption processes on encysted cells of Haematococcus pluvialis: effects of astaxanthin recovery and implications for bio-availability. J appl phycol 13:19–24

    Article  Google Scholar 

  44. Moen E (2008) Biological degradation of brown seaweeds. The potential of marine biomass for anaerobic biogas production. Scottish Association for Marine Science, Oban, Scotland

    Google Scholar 

  45. Molina Grima E, Belarbi E-H, Acien Fernandez FG, Robles Medina A, Chisti Y (2003) Recovery of microalgal biomass and metabolites: process options and economics. Biotechnol Adv 20:491–515

    Article  CAS  Google Scholar 

  46. Nagle N, Lemke P (1990) Production of methyl ester fuel from microalgae. Appl Biochem Biotechnol 24:355–361

    Article  Google Scholar 

  47. Nguyen MT, Choi SP, Lee J, Lee JH, Sim SJ (2009) Hydrothermal acid pretreatment of Chlamydomonas reinhardtii biomass for ethanol production. J Microbiol Biotechnol 19(2):161

    Article  CAS  Google Scholar 

  48. Ota M, Kato Y, Watanabe H, Watanabe M, Sato Y, Smith R, Inomata H (2009) Fatty acid production from a highly CO2 tolerant alga, Chlorocuccum littorale, in the presence of inorganic carbon and nitrate. Bioresour Technol 100:5237–5242

    Article  CAS  Google Scholar 

  49. Pimentel D, Patzek TW (2008) Ethanol production: energy and economic issues related to U.S. and Brazilian sugarcane. In: Pimentel D (ed) Biofuels, solar, wind as renewable energy systems. Springer, Netherlands, pp 357–371

    Chapter  Google Scholar 

  50. Pourmortazavi SM, Hajimirsadeghi SS (2007) Supercritical fluid extraction in plant essential and volatile oil analysis—review. J Chromatogr A 1163:2–24

    Article  CAS  Google Scholar 

  51. Ramadan MF, Asker MHS, Ibrahim ZK (2008) Functional bioactive compounds and biological activities of Spirulina platensis lipids. Czech J Food Sci 26(3):211–222

    CAS  Google Scholar 

  52. Saha BC, Iten LB, Cotta MA, Wu YV (2005) Dilute acid pretreatment, enzymatic saccharification and fermentation of wheat straw to ethanol. Proc Biochem 40:3693–3700

    Article  CAS  Google Scholar 

  53. Sajilata MG, Singhal RS, Kamat MY (2008) Supercritical CO2 extraction of γ-linolenic acid (GLA) from Spirulina platensis ARM 740 using response surface methodology. J Food Eng 84:321–326

    Article  CAS  Google Scholar 

  54. Schenk P, Thomas-Hall S, Stephens E (2008) Second generation biofuels: high efficiency microalgae for biodiesel production. BioEnerg Res 1:20–43

    Article  Google Scholar 

  55. Schwartzberg HG (1997) Mass transfer in a countercurrent, supercritical extraction system for solutes in moist solids. Chem Eng Commun 157:1–22

    Article  CAS  Google Scholar 

  56. Sheehan J, Dunahay T, Benemann J, Roessler P (1998) A look back at the US Department of Energy’s Aquatic Species Program – biodiesel from algae. In: Close-Out Report by National Renewable Energy Laboratory, Golden, Colorado. Report no.: NREL/TP-580-24190. http://www.nrel.gov/docs/legosti/fy98/24190.pdf. Accessed 10 April 2009

  57. Shenk P, Thomas-Hall S, Stephens E, Marx U, Mussgnug J, Posten C (2008) Second generation biofuels: high-efficiency microalgae for biodiesel production. BioEnerg Res 1(1):20–43

    Article  Google Scholar 

  58. Sun Y, Cheng J (2002) Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresour Technol 83:1–11

    Article  CAS  Google Scholar 

  59. Taylor LT (1996) Supercritical fluid extraction. Wiley, New York

    Google Scholar 

  60. Usov AI, Smirnova GP, Klochkova NG (2001) Polysaccharides of algae: 55 polysaccharide composition of several brown algae from Kamchatka Russian. J Bioorgan Chem 27:395–399

    Article  CAS  Google Scholar 

  61. Volkman JK, Jeffrey SW, Nichols PD, Rogers GI, Garland CD (1989) Fatty acid and lipid composition of 10 species of microalgae used in mariculture. J Exp Mar Biol Ecol 128:219–240

    Article  CAS  Google Scholar 

  62. Wang L, Weller CL (2006) Recent advances in extraction of nutraceuticals from plants. Trends Food Sci Tech 17:300–312

    Article  CAS  Google Scholar 

  63. Wayman M (1969) Cellulases and their applications. American Chemical Society, Washington, DC

    Google Scholar 

  64. Widjaja A, Chien C-C, Ju Y-H (2009) Study of increasing lipid production from fresh water microalgae Chlorella vulgaris. J Taiwan Inst Chem Eng 40(1):13–20

    Article  CAS  Google Scholar 

  65. Zhang X, Shen Y, Shi W (2010) Ethanolic cofermentation with glucose and xylose by the recombinant industrial strain Saccharomyces cerevisiae NAN-127 and the effect of furfural on xylitol production. Bioresour Technol 101:7104–7110

    Google Scholar 

  66. Zhu JY, Pan XJ (2010) Woody biomass pretreatment for cellulosic ethanol production: technology and energy consumption evaluation. Bioresour Technol 101(13):4992–5002

    Article  CAS  Google Scholar 

Download references

Acknowledgement

This work was supported by an Australian Research Council (ARC) Linkage grant between Bio-Fuel Pty Ltd (Victoria, Australia) and Monash University Department of Chemical Engineering (Victoria, Australia).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Michael K. Danquah .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer Science+Business Media New York

About this chapter

Cite this chapter

Halim, R., Harun, R., Webley, P.A., Danquah, M.K. (2013). Bioprocess Engineering Aspects of Biodiesel and Bioethanol Production from Microalgae. In: Lee, J. (eds) Advanced Biofuels and Bioproducts. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-3348-4_25

Download citation

Publish with us

Policies and ethics