Abstract
Cyclophosphamide, an important cancer chemotherapeutic agent, has been shown to be effective against some types of cancer (Hilton, 1984a; Manthey and Sladek, 1989). However, both natural and acquired resistance to cyclophosphamide limits its utility in the clinic. Several investigators have observed a dramatic increase (about 200-fold) of cytosolic aldehyde dehydrogenase (ALDH, EC 1.2.1.3) activity in a cyclo-phosphamide-resistant murine leukemia cell line. It has been proposed that ALDH which can oxidize aldophosphamide, an intermediate metabolite produced from cyclophosphamide, prevents the generation of tissue toxic phosphamide mustard within cells (Manthey and Sladek, 1989; Russo et al., 1989). The drug resistance of the murine cell line with high ALDH activity can be explained by this mechanism.
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References
Bannasch, P., Hacker, H. J., Klimek, F., and Mayer, P., 1983, Hepatocellular glycogenesis and related pattern of enzymatic changes during hepatocarcinogenesis, Adv. Enzyme Reg., 22: 97.
Chirgwin, J. J., Przbyla, A. E., MacDonald, R. J., and Rutter, W. J., 1979, Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease, Biochem., 18: 5294.
Crook, T. R., Souhami, R. L., Whyman, G. D., and McLean, A. E.M., 1986, Glutathione depletion as a determinant of sensitivity of human leukemic cells to cyclophosphamide, Cancer Res., 46: 5035.
Dunn, T.J., Koleske, A.J., Lindahl, R., and Pitot, H.C. 1989, Phenobarbital-inducible aldehyde dehydrogenase in the rat: cDNA sequence and regulation of the mRNA by phenobarbital in responsive rats. J. Biol. Chem. 264: 13057.
Eva, A., Robbins, K. C., Andersen, P. R., Srinivasan, A., Tronick, S. R., Reddy, E. P., Ellmore, N. W., Galen, A. T., Lantenberger, J. A., Papas, T. S., Westin, E. H., Wong-Stall, F., Gallo, R. C., and Anderson, S. A., 1982, Cellular genes analogous to retroviral onc genes as transcribed in human tumor cells, Nature, 295: 116.
Green, J. A., Vistica, D. T., Young, R. C., Hamilton, T. C., Rogan, A. M., and Ozols, R. F., 1984, Melphalan resistance in human ovarian cancer: Characterization of drug-resistant cell lines and potentiation of melphalan cytotoxicity by glutathione depletion, Cancer Res., 44: 5427.
Greenfield, N. J., and Pietruszko, T., 1977, Two aldehyde dehydrogenases from human liver: Isolation via affinity chromatography and characterization of the isozymes, Bioch. Bioshys. Acta, 483: 35.
Hilton, J., 1984a, Role of aldehyde dehydrogenase in cyclophosphamide-resistant L1210 leukemia, Cancer Res. 44: 5156.
Hilton, J., 1984b, Deoxyribonucleic acid crosslinking by 4-hydro-peroxycyclo-phosphamide in cyclophosphamide-sensitive and resistant L1210 cells, Bioch. Pharm., 33: 1867.
Hsu, L. C., Tani, K., Fujiyoshi, T., Kurachi, K., and Yoshida, A., 1985, Cloning of cDNAs for human aldehyde dehydrogenases 1 and 2, Prog. Natl. Acad. Sci. USA. 82: 2703.
Ikawa, M., Impraim, C. C., Wang, G., and Yoshida, A., 1983, Isolation and characterization of aldehyde dehydrogenase isozymes from usual and atypical human livers, J. Biol. Chem., 258: 6282.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J., 1951, Protein measurement with the Folin phenol reagent, J. Biol. Chem., 193: 265.
Lu, Y., Han, J., and Scanlon, K. J., 1988. Biochemical and molecular properties of cisplatin-resistant A2780 cells grown in folinic acid, J. Biol. Chem., 263: 4891.
Manthey, C. L., and Sladek, N. E., 1989, Aldehyde dehydrogenasecatalyzed bioinactivation of cyclophosphamide, in “Enzymology and Molecular Biology of Carbonyl Metabolism,” H. Weiner, ed., Alan R. Liss, New York, p. 49.
Michelson, A. M., Markham, A. F., and Orkin, S. H., 1983, Isolation and DNA sequence of a full-length CDNA clone for human X chromosome-encoded phosphoglycerate kinase, Proc. Natl. Acad. Sci. USA. 80: 472.
Radin, A. I., Zhoa X-L., Woo, T. H., Colvin, M., and Hilton, J., 1991, Structure and expression of the cytosolic aldehyde dehydrogenase gene in cyclophosphamide-resistant murinBe leukemia L1210 cells, Biochem. Pharm., 42: 1933.
Russo, J. E., Hilton, J., and Colvin, O. M., 1989, The role of aldehyde dehydrogenase isozymes in cellular resistance to the alkylating agent cyclophosphamide, in “Enzymology and Molecular Biology of Carbonyl Metabolism,” H. Weiner, ed., Alan R. Liss, New York, p. 65.
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., and Erlich, H. A., 1988, Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase, Science. 239: 487.
Sambrook, J., Fritsch, E. F., and Maniatis, T., 1989, “Molecular Cloning: A Laboratory Manual,” 2nd ed., Cold Spring Harbor Lab Press, New York.
Takizawa, T., Huang, I-Y., Ikuta, T., and Yoshida, A., 1986, Human glucose-6-phosphate dehydrogenase: Primary structure and CDNA cloning, Proc. Natl. Acad. Sci. USA. 83: 4157.
Tani, K., Takizawa, T., and Yoshida, A., 1985, Normal mRNA content in a phosphoglycerate kinase variant with severe enzyme deficiency, Am. J. Hum. Genet., 37: 931.
Tompkins, W. A. F., Watrach, A. M., Schmale, J. D., Schultz, R. M., and Harris, J. A., 1974, Cultural and genetic properties of newly established cell strains derived from adenocarcinomas of the human colon and rectum, J. Natl. Cancer Inst., 52: 1101.
Weber, G., Prajda, N., and Williams, J. C., 1975, Biochemical strategy of the cancer cell: malignant transformation-linked enzymatic imbalance, Adv. Enzyme Reg., 13: 3.
Yoshida, A., 1966, Glucose-6-phosphate dehydrogenase of human erythrocytes, J. Biol. Chem., 241: 4966.
Yoshida, A., and Watanabe, S., 1972, Human phosphoglycerate kinase I: Crystallization and characterization of normal enzyme, J. Biol, Chem., 247: 440.
Yoshida, A., Hsu, L.C., and Yasunami, M., 1991, Genetics of human alcohol-metabolizing enzymes, Prog. Nucleic Acid Res. Mol. Biol., 40: 255.
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Yoshida, A., Davé, V., Han, H., Scanlon, K.J. (1993). Enhanced Transcription of the Cytosolic ALDH Gene in Cyclophosphamide Resistant Human Carcinoma Cells. In: Weiner, H., Crabb, D.W., Flynn, T.G. (eds) Enzymology and Molecular Biology of Carbonyl Metabolism 4. Advances in Experimental Medicine and Biology, vol 328. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2904-0_8
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