Abstract
Water soluble nucleic acid analogs were prepared by grafting uracil, thymine, 5-fluorouracil, hypoxanthine, cytosine, and adenine derivatives containing a hydroxy group onto linear poly (ethyleneimine). These polymers contained about 90 unit% of the nucleic acid bases, and were easily soluble in water at neutral pH resign. The analogs were found to form complementary polymer complexes with each other and with polynucleotides. The interaction study was made on these polymers, and with polynucleotides; poly (uridylic acid) poly (U), poly (adenylic acid) poly (A), poly (inosinic acid) poly (I) and poly (cytidylic acid) poly(C) in water. They were found to form polymer complexes with polynucleotide by specific base-base interaction in aqueous solution.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
R. M. Ottenbrite, “Anionic Polymeric Drugs,” L. G. Donaruma, R. M. Ottenbrite, O. Vogl, Eds., John Wiley & Sons, Inc., p. 1, (1980).
J. H. Park, M. A. Gallin, A. Billiau, and S. Baron, Arch. Ophthal., 81, 840 (1969).
H. B. Levy, J. Bioactive and Compatible Polymers, 1, 348 (1986).
A. K. Field, A. A. Tytell, G. P. Lampson, and M. R. Hillman, Proc. Nat. Acad. Sci. U.S.A., 58, 1004 (1967).
M. M. Nemes, A. A. Tytell, G. P. Lampson, A. K. Field, and M. R. Hillman, Proc. Soc. Exp. Biol. Med., 132, 776 (1969).
V. DeVita, G. Canellos, P. Carbone, H. Levy, and H. Graenick, Proc. Am. Assoc. Cancer Res., 11, 21 (1970).
M. Absher and W. R. Stinebring, Nature, 223, 715 (1969).
T. Sato, K. Kojima, T. Ihda, J. Sunamoto, and R. M. Ottenbrite, J. Bioactive and Compatible Polymers, 1, 448 (1986).
K. Takemoto and Y. Inaki, Adv. Polym. Sci., 41, 1 (1981).
J. Pitha, Adv. Polym. Sci., 50, 1 (1983).
A. G. Ludwick, K. S. Robinson, and J. McCloud, Jr., J. Polym. Sci. Polym. Symp., 74, 55 (186).
Y. Inaki and K. Takemoto, “Current Topics in Polymer Science,” Volume I, R. M. Ottenbrite, L. A. Utracki, and S. Inoue, Eds., Hanser Publisher, p. 79, (1987).
K. Takemoto and Y. Inaki, “Functional Monomers and Polymers,” K. Takemoto, Y. Inaki, and R. M. Ottembrite, Eds., Marcel Dekker, Inc., p. 149, (1987).
J. Sunamoto and K. Takemoto, to be published.
T. Wada, Y. Inaki, and K. Takemoto, Polym. J., 21, 11 (1989).
T. Wada, Y. Inaki, and K. Takemoto, Polym. J., 20, 1059 (1988).
T. Wada, Y. Inaki, and K. Takemoto, J. Bioactive and Compatible Polymers, 4, 25 (1989).
G. J. Thomas, Jr., and Y. Kyogoku, J. Am. Chem. Soc., 89, 4170 (1967).
J. Pitha, P. M. Pitha, and E. Stuart, Biochemistry, 10, 4595 (1971);
J. Pitha, P. M. Pitha, and E. Stuart, Biopolymers, 9, 965 (1970);
C. G. Overberger, Y. Inaki, and Y. Nambu, J. Polym. Sci. Polym. Chem. Ed., 17, 1759 (1979).
C. G. Overberger and Y, Inaki, J. Polym. Sci. Polym. Chem. Ed., 17, 1739 (1979).
Y. Inaki, Y. Sakuma, Y. Suda and K. Takemoto, J. Polym. Sci. Polym. Chem. Ed., 20, 1917 (1982).
D. Thiele, W. Guschlbauer, and A. Faver, Biochim. Biophys. Acta, 272, 22 (1972).
K. Fujioka, Y. Baba, A. Kagemoto, and R. Fujishiro, Polym. J., 12, 843 (1980).
S. Fang, Y. Inaki, and K. Takemoto, J. Polym. Sci. Polym. Chem. Ed., 22, 2455 (1984).
Y. Inaki, S. Sugita, T. Takahara, and K. Takemoto, J. Polym. Sci. Polym. Chem. Ed., 24, 3201 (1986).
P. O. P. Ts’o, Ed., “Basic Principles of Nucleic Acid Chemistry,” Vols. 1 and 2, Academic Press, New York (1974).
S. Higuchi, Biopolymers, 23, 493 (1984).
S. Higuchi, Biopolymers, 23, 831 (1984).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer Science+Business Media New York
About this chapter
Cite this chapter
Takemoto, K., Wada, T., Mochizuki, E., Inaki, Y. (1991). Synthesis and Interaction of Water Soluble Nucleic Acid Analogs. In: Gebelein, C.G. (eds) Biotechnology and Polymers. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3844-8_3
Download citation
DOI: https://doi.org/10.1007/978-1-4615-3844-8_3
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6715-4
Online ISBN: 978-1-4615-3844-8
eBook Packages: Springer Book Archive