Abstract
Investigations of bacteriophage infection of Eschenichia coli have established a role for the DNA-dependent RNA polymerase in the regulation of gene expression. It is now generally accepted that host E. coli RNA polymerase transcribes at least a part of the phage genome (the ‘early’ regions) whereas a modified host RNA polymerase (in the case of T4) or even a new phage-specified polymerase (as in the case of T7) transcribes the ‘late’ regions (Travers, 1971; Bautz, 1972). These observations suggest that the host E. coli RNA polymerase, the modified T4 polymerase and the new phage-specified polymerase recognize different nucleotide sequences and thus bind to and transcribe from different initial binding sites (promoter sites) on the phage DNA. Thus at least one “positive” control mechanism resides in the ability of different polymerase species to recognize different promoter sites.
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Flint, S.J., de Pomerai, D.I., Chesterton, C.J., Butterworth, P.H.W. (1973). Studies on the Template Specificities of Eukaryotic DNA-Dependent RNA Polymerases in Vitro . In: Hamkalo, B.A., Papaconstantinou, J. (eds) Molecular Cytogenetics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7479-8_14
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DOI: https://doi.org/10.1007/978-1-4615-7479-8_14
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