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Fusion of Microbial Protoplasts: Problems and Perspectives

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Genetic Engineering of Microorganisms for Chemicals

Part of the book series: Basic Life Sciences ((BLSC))

Abstract

Induced fusion of protoplasts is a relatively new way of genetic manipulation in microbial systems. The individual steps of the manipulation are shown in the scheme of Figure 1. The rigid cell wall of bacteria, fungi, yeast, and algae (as well as of plant cells) can be removed without influencing the viability and integrity of the cell. The wall-less microorganism is surrounded by a plasma membrane, in hypertonic media it is spherical in shape, and is called a protoplast. The protoplasts, under appropriate conditions, do resynthesize cell wall material and revert to the original form with characteristic morphology.

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Authors and Affiliations

  1. Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary

    Lajos Alföldi

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  1. Lajos Alföldi
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Editors and Affiliations

  1. Associated Universities, Inc., Washington, D.C., USA

    Alexander Hollaender

  2. Department of Microbiology, University of Illinois, Champaign, Urbana, USA

    Ralph D. DeMoss

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© 1982 Plenum Press, New York

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Alföldi, L. (1982). Fusion of Microbial Protoplasts: Problems and Perspectives. In: Hollaender, A., DeMoss, R.D., Kaplan, S., Konisky, J., Savage, D., Wolfe, R.S. (eds) Genetic Engineering of Microorganisms for Chemicals. Basic Life Sciences. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4142-0_7

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  • DOI: https://doi.org/10.1007/978-1-4684-4142-0_7

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-4144-4

  • Online ISBN: 978-1-4684-4142-0

  • eBook Packages: Springer Book Archive

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