Abstract
This discussion is about tools to analyze the complex relationship between chemicals and mutation in a relatively simple biological system: the exponentially growing single cell population. Three assumptions are made about this relationship: that some DNA adducts are premutagenic lesions, that adducted DNA may be repaired, misrepaired or unrepaired at the time of DNA synthesis and that unrepaired DNA may be replicated, misreplicated or unreplicated at the time of mitosis. These three assumptions lead to the definition of variables ascertainable by experimental measurement which, in turn, lead to explicit formulae which should be useful as guides in the analysis of dose(adduct) -response(mutation) relations.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
DeLuca, J.G., Kaden, D.A., Krolewski, J.J., Skopek, T.R. and Thilly, W.G. (1977) Comparative mutagenicity of ICR-191 to S. typhimurium and diploid human lymphoblasts. Mutat. Res, 46: 11–18.
Elkind, M.M. and Sutton,H. (1959) X-ray camage and recovery in mammalian cells in culture. Nature, 184: 1293–1295.
Elkind, M.M. and Whitmore,G.F. (1967) Radiobiology of Cultured Mammalian Cells, Gordon and Breach, New York.
Elkind, M.M. (1977) The Initial part of the survival curve. Implications for low-dose, low-dose-rate radiation responses. Radiation Res. 71: 9–23.
Haynes, R.H. and Eckardt, F. (1979) Analysis of dose-response patterns in mutation research. Canad. J. Genetics and Cytology, 21: 277–302.
Kaufmann, W.K., Cleaver, J.E. and Painter, R.B.,(1980) Ultraviolet radiation inhibits replicon initiation in S phase human cells. Biochem. Biophys. Acta, 608: 191–195.
Park, S.D. and Cleaver, J.E. (1979) Recovery of DNA synthesis after ultraviolet irradiation of xeroderma pigmentosum cells depends on excision repair and is blocked by caffeine. Nucleic Acids Res., 6: 1151–1159.
Penman, B.W., Kaden, D.A., Liber, H.L., Skopek, T.R. and Thilly, W.G. (1980) Perylene is a more potent mutagen than benzo(a)pyrene for S. typhimurium. Mutation Res. 77: 271–277.
Thilly, W.G., DeLuca, J.G., Hoppe, H. IV and Penman, B.W. (1976) Mutation of human lymphoblasts by methylnitrosourea. Chem.- Biol. Interactions, 15: 33–50.
Thompson, L.H., Rubin, J.S., Cleaver, J.E., Whitmore, G.F. and Brookman, K. (1980) A screening method for isolating DNA repair-deficient mutants of CHO cells. Somatic Cell Genet. 6: 391–405.
Wogan, G.N., Croy, R.G., Essigman, J.D., Groopman, J.D., Thilly, W.G., Skopek, T.R. and Liber, H.L. (1979) Mechanisms of action of aflatoxin B1 and sterigmatocystin: Relationships of macro-molecular binding to carcinogenicity and mutagenicity. In Environmental Carcinogenesis, Ed. by P. Emmelot and E. Kriek. Elsevier/North Holland Biomedical Press, Amsterdam, pp. 97–121.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1983 Plenum Press, New York
About this chapter
Cite this chapter
Thilly, W.G. (1983). Analysis of Chemically Induced Mutation in Single Cell Populations. In: Lawrence, C.W. (eds) Induced Mutagenesis. Basic Life Sciences, vol 15. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4382-0_14
Download citation
DOI: https://doi.org/10.1007/978-1-4684-4382-0_14
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4384-4
Online ISBN: 978-1-4684-4382-0
eBook Packages: Springer Book Archive