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Determination of Urinary Oxalate by Reversed-Phase ION-Pair “High-Performance” Liquid Chromatography

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Urolithiasis and Related Clinical Research

Abstract

Calcium oxalate is an important constituent of the majority of renal stones. Thus measurement of urinary oxalate has become essential in the basic evaluation of the urolithiasis patient. However, the large number of published methods indicate the presence of considerable methodological difficulties1. In this paper we describe our recently developed method for determination of urinary oxalate, based on a reversed-phase ion-pair “high-performance” liquid chromatography (HPLC)2. We also describe a comparison performed between our HPLC method, the colorimetric method of Hodgkinson and Williams3, and a commercial enzymatic method4.

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References

  1. W. G. Robertson and A. Rutherford, Scand. J. Urol. Nephrol. Suppl. 53:85 (1981).

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  2. L. Larsson, B. Libert, and M. Asperud, Clin. Chem. 28:2274 (1982)

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  3. A. Hodgkinson and A. Williams, Clin. Chim. Acta 36:127 (1972)

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  4. Sigma Technical Bulletin No. 590, April 1983.

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  5. D. Rodbard, Clin. Chem. 20:1265 (1974).

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  6. J. E. Zerwekh, E. Drake, J. Gregory, D. Griffith, A. F. Hofmann, M. Menon, and C. Y. C. Pak, Clin. Chem. 29:1977 (1983).

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  7. D. S. Scurr, N. Januzovic, A. Smith, and W. G. Robertson, Urol. Res. 12:90 (1984).

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Author information

Authors and Affiliations

  1. Dept. of Clinical Chemistry, Linköping University, Linköping, Sweden

    L. Larsson & B. Libert

  2. Dept. of Plant Breeding, Swedish University of Agricultural Sciences, Uppsala, Sweden

    M. Asperud

Authors
  1. L. Larsson
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  2. B. Libert
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  3. M. Asperud
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Editor information

Editors and Affiliations

  1. University Hospital, Erlangen, Federal Republic of Germany

    Paul O. Schwille

  2. Mayo Clinic, Rochester, Minnesota, USA

    Lynwood H. Smith

  3. The General Infirmary, Leeds, England

    William G. Robertson

  4. University Hospital, Bonn, Federal Republic of Germany

    Winfried Vahlensieck

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© 1985 Plenum Press, New York

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Larsson, L., Libert, B., Asperud, M. (1985). Determination of Urinary Oxalate by Reversed-Phase ION-Pair “High-Performance” Liquid Chromatography. In: Schwille, P.O., Smith, L.H., Robertson, W.G., Vahlensieck, W. (eds) Urolithiasis and Related Clinical Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7272-1_141

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  • DOI: https://doi.org/10.1007/978-1-4684-7272-1_141

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-7274-5

  • Online ISBN: 978-1-4684-7272-1

  • eBook Packages: Springer Book Archive

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