Skip to main content

The Nature and Function of the Microbicidal Oxidase System of Neutrophils

  • Chapter
Biochemistry and Function of Phagocytes

Abstract

Stimulated neutrophils demonstrate a burst of nonmitochondrial respiration.1,2 This appears to be important for the killing of certain bacteria which are killed less efficiently under anaerobic conditions,3 or by cells from patients with Chronic Granulomatous Disease (CGD) which fail to demonstrate this respiratory burst.4

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Baldridge C.W. and Gerard R.W. (1933) The extra respiration of phagocytosis. Am. J. Physiol. 103, 235–236.

    CAS  Google Scholar 

  2. Sbarra A.J. and Karnovsky M.L. (1959) The biochemical basis of phagocytosis. 1. Metabolic changes during the ingestion of particles by polymorphonuclear. J. Biol. Chem. 234, 1355–1362.

    PubMed  CAS  Google Scholar 

  3. Salvaraj R.J. and Sbarra A.J. (1966) Relationship of glycolytic and oxidative metabolism to particle entry and destruction in phagocytosing cells. Nature 211, 1272–1276.

    Article  Google Scholar 

  4. Holmes B., Page A.R. and Good R.A. (1967) Studies of the metabolic activity of leukocytes from patients with a genetic abnormality of phagocytic function. J. Clin. Invest. 46, 1422–1432.

    Article  PubMed  CAS  Google Scholar 

  5. Cagan R.H. and Karnovsky M.L. (1964) Enzymic basis of the respiratory stimulation during phagocytosis. Nature 204, 255–257.

    Article  PubMed  CAS  Google Scholar 

  6. Iyer G.Y.N. and Quastel J.H. (1963) NADPH and NADH oxidation by guinea pig polymorphonuclear leukocytes. Canadian Journal of Biochemistry and Physiology, 41, 427–434.

    Article  PubMed  CAS  Google Scholar 

  7. Rossi F. and Zatti M. (1964) Changes in the metabolic pattern of polymorphonuclear leukocytes during phagocytosis. Brit. J. Exp. Path. 45, 548–559.

    PubMed  CAS  Google Scholar 

  8. Gray G.R., Klebanoff S.J., Stamatoyannopoulos G.,Austin T., Naiman S.C., Yoshida A., Kliman M.R. and Robinson G.C.F. (1973) Neutrophil dysfunction, chronic granulomatous disease and non-spherocytic haemolytic anaemia caused by complete deficiency of glucose-6-phosphate dehydrogenase. Lancet 2, 530–534.

    Article  PubMed  CAS  Google Scholar 

  9. Segal A.W. and Jones, O.T.G. (1978) A novel cytochrome b system in phagocytic vacuoles from human granulocytes. Nature 276, 515–517.

    Article  PubMed  CAS  Google Scholar 

  10. Segal A.W. and Jones O.T.G. (1979) The subcellular distribution and some properties of the cytochrome b component of the microbicidal oxidase system of human neutrophils. Biochem. J. 180, 33–44.

    Google Scholar 

  11. Segal A.W. and Jones O.T.G. (1980) Rapid incorporation of the human neutrophil plasma membrane cytochrome b into phagocytic vacuoles. Biochem. Biophys. Res. Commun. 92, 710–715.

    Article  PubMed  CAS  Google Scholar 

  12. Segal A.W. and Jones O.T.G. (1979) Reduction and subsequent oxidation of a cytochrome b of human neutrophils after stimulation with phorbol myristate acetate. Biochem. Biophys. Res. Commun. 88, 130–134.

    Article  PubMed  CAS  Google Scholar 

  13. Segal A.W. and Jones O.T.G. (1980) Absence of cytochrome b reduction in stimulated neutrophils from both female and mâle patients with Chronic Granulomatous Disease. FEES Letters 110, 111–114.

    Article  CAS  Google Scholar 

  14. Ohkuma S. and Poole B. (1978) Fluorescence probe measurement of the intralysosomal pH in living cells and the perturbation of pH by various agents. Proc. Natl. Acad. Sci. USA. 75, 3327–3331.

    Article  PubMed  CAS  Google Scholar 

  15. Mandell G.L. (1970) Intraphagosomal pH of human polymorphonuclear neutrophils Proc. of the Soc. for Exp. Biol. and Med. 134, 447–449.

    CAS  Google Scholar 

  16. Quie P.G., White J.G., Holmes B. and Good R.A. (1967) In vitro bactericidal capacity of human polymorphonuclear leukocytes: Diminished activity inehronic granulomatous disease of childhood. J. Clin. Invest. 46, 668–679.

    Article  PubMed  CAS  Google Scholar 

  17. Odeberg H. and Olsson I. (1975) Anti-bacterial activity of cationic proteins from human granulocytes. J. Clin. Invest. 56, 1118–1124.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1982 Plenum Press, New York

About this chapter

Cite this chapter

Segal, A.W. et al. (1982). The Nature and Function of the Microbicidal Oxidase System of Neutrophils. In: Rossi, F., Patriarca, P. (eds) Biochemistry and Function of Phagocytes. Advances in Experimental Medicine and Biology, vol 141. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8088-7_38

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-8088-7_38

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-8090-0

  • Online ISBN: 978-1-4684-8088-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics