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Sample Types, Collection, and Transport for Influenza A Viruses of Swine

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Book cover Animal Influenza Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1161))

Abstract

Detection of influenza A virus (IAV), viral antigen, nucleic acid, or antibodies in swine is dependent upon the collection of the appropriate sample type, the quality of the sample, and the proper storage and handling of the sample. The diagnostic tests to be performed should be considered prior to sample collection. Sera are acceptable samples for ELISA or hemagglutination inhibition tests, but not for real-time RT-PCR. Likewise, swabs and/or tissues are acceptable for real-time RT-PCR and virus isolation. The sample type will also depend on the age of swine that are being tested; oral fluids can be successfully collected from weaned pigs usually greater than 3 weeks of age, whereas nasal swabs should be collected from suckling pigs in the first weeks of life. This chapter outlines the collection of different specimen types and procedures for proper specimen handling.

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References

  1. Abdelwhab EM, Luschow D, Harder TC, Hafez HM (2011) The use of FTA® filter papers for diagnosis of avian influenza virus. J Virol Methods 174:120–122

    Article  CAS  PubMed  Google Scholar 

  2. Kraus RH, van Hooft P, Waldenstrom J, Latorre-Margalef N, Ydenberg RC, Prins HH (2011) Avian influenza surveillance with FTA cards: field methods, biosafety, and transportation issues solved. J Vis Exp 54:2832

    PubMed  Google Scholar 

  3. Haines DM, Waters EH, Clark EG (1993) Immunohistochemical detection of swine influenza A virus in formalin-fixed and paraffin-embedded tissues. Can J Vet Res 57:33–36

    CAS  PubMed Central  PubMed  Google Scholar 

  4. Vincent LL, Janke BH, Paul PS, Halbur PG (1997) A monoclonal-antibody-based immunohistochemical method for the detection of swine influenza virus in formalin-fixed, paraffin-embedded tissues. J Vet Diagn Invest 9:191–195

    Article  CAS  PubMed  Google Scholar 

  5. Skibbe D, Zhou EM, Janke BH (2004) Comparison of a commercial enzyme-linked immunosorbent assay with hemagglutination inhibition assay for serodiagnosis of swine influenza virus (H1N1) infection. J Vet Diagn Invest 16:86–89

    Article  PubMed  Google Scholar 

  6. Leuwerke B, Kitikoon P, Evans R, Thacker E (2008) Comparison of three serological assays to determine the cross-reactivity of antibodies from eight genetically diverse U.S. swine influenza viruses. J Vet Diagn Invest 20:426–432

    Article  PubMed  Google Scholar 

  7. Yoon KJ, Janke BH, Swalla RW, Erickson G (2004) Comparison of a commercial H1N1 enzyme-linked immunosorbent assay and hemagglutination inhibition test in detecting serum antibody against swine influenza viruses. J Vet Diagn Invest 16:197–201

    Article  PubMed  Google Scholar 

  8. Heinen PP, de Boer-Luijtze EA, Bianchi AT (2001) Respiratory and systemic humoral and cellular immune responses of pigs to a heterosubtypic influenza A virus infection. J Gen Virol 82:2697–2707

    CAS  PubMed  Google Scholar 

  9. Heinen PP, Rijsewijk FA, de Boer-Luijtze EA, Bianchi AT (2002) Vaccination of pigs with a DNA construct expressing an influenza virus M2-nucleoprotein fusion protein exacerbates disease after challenge with influenza A virus. J Gen Virol 83:1851–1859

    CAS  PubMed  Google Scholar 

  10. Van Reeth K, Labarque G, De Clercq S, Pensaert M (2001) Efficacy of vaccination of pigs with different H1N1 swine influenza viruses using a recent challenge strain and different parameters of protection. Vaccine 19:4479–4486

    Article  PubMed  Google Scholar 

  11. Van Reeth K, Gregory V, Hay A, Pensaert M (2003) Protection against a European H1N2 swine influenza virus in pigs previously infected with H1N1 and/or H3N2 subtypes. Vaccine 21:1375–1381

    Article  PubMed  Google Scholar 

  12. Van Reeth K, Labarque G, Pensaert M (2006) Serological profiles after consecutive experimental infections of pigs with European H1N1, H3N2, and H1N2 swine influenza viruses. Viral Immunol 19:373–382

    Article  PubMed  Google Scholar 

  13. Humphrey SP, Williamson RT (2001) A review of saliva: normal composition, flow, and function. J Prosthet Den 85:162–169

    Article  CAS  Google Scholar 

  14. Kaufman E, Lamster IB (2002) The diagnostic applications of saliva: a review. Crit Rev Oral Biol Med 13:197–212

    Article  PubMed  Google Scholar 

  15. Detmer SE, Patnayak DP, Jiang Y, Gramer MR, Goyal SM (2011) Detection of influenza A virus in porcine oral fluid samples. J Vet Diagn Invest 23:241–247

    Article  PubMed  Google Scholar 

  16. Olsen CW, Brown IH, Easterday BC, Van Reeth K (2006) Swine influenza. In: Straw BE et al (eds) Diseases of swine, 9th edn. Blackwell, Ames, IA, pp 469–482

    Google Scholar 

  17. Rossow KD, Yeske P, Goyal SM, Webby RJ, Collins JE (2003) Diagnostic investigation of unexpected serology results for swine influenza virus (SIV) and porcine reproductive and respiratory syndrome virus (PRRSV). J Swine Health Prod 11:33–35

    Google Scholar 

  18. Webster RG, Cox N, Stohr K (2002) WHO manual on animal influenza diagnosis and surveillance (cited 4 Aug 2013). Available from http://www.who.int/csr/resources/publications/influenza/whocdscsrncs20025rev.pdf

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Authors and Affiliations

  1. Veterinary Diagnostic Laboratory, University of Minnesota, 1333 Gortner Avenue, Saint Paul, MN, 55108, USA

    Marie R. Culhane

  2. Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada

    Susan E. Detmer

Authors
  1. Marie R. Culhane
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  2. Susan E. Detmer
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Corresponding author

Correspondence to Marie R. Culhane .

Editor information

Editors and Affiliations

  1. Exotic and Emerging Avian Viral Diseases Unit, Southeast Poultry Research Laboratory, US Department of Agriculture, Agricultural Research Service, Athens, Georgia, USA

    Erica Spackman

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Culhane, M.R., Detmer, S.E. (2014). Sample Types, Collection, and Transport for Influenza A Viruses of Swine. In: Spackman, E. (eds) Animal Influenza Virus. Methods in Molecular Biology, vol 1161. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0758-8_21

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  • DOI: https://doi.org/10.1007/978-1-4939-0758-8_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0757-1

  • Online ISBN: 978-1-4939-0758-8

  • eBook Packages: Springer Protocols

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