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Solid State NMR of Lipid Model Membranes

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Methods in Membrane Lipids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1232))

Abstract

In this chapter we describe the use of solid state nuclear magnetic spectroscopy to study the structure of lyotropic phases and lipid model membranes and show its ability to probe, site specifically, at a sub-Ångstrom resolution. Here, we demonstrate the immense versatility of the technique and its ability to provide information on the different liquid crystalline phases present. A multinuclear for example 31P, 1H, and 13C approach is able to elucidate both the structure and dynamics over a wide variety of timescales. This coupled with a non-perturbing label 2H is able to provide information such as the order parameters for a wide variety of different liquid phases.

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Author information

Authors and Affiliations

  1. Department of Chemistry, Imperial College London, South Kensington Campus, London, SW7 2AZ, UK

    Arwen I. I. Tyler, James A. Clarke, John M. Seddon & Robert V. Law

Authors
  1. Arwen I. I. Tyler
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  2. James A. Clarke
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  3. John M. Seddon
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  4. Robert V. Law
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Corresponding authors

Correspondence to Arwen I. I. Tyler or Robert V. Law .

Editor information

Editors and Affiliations

  1. Department of Physics and Randall Division of Cell and Molecular Biophysics, King’s College London, London, United Kingdom

    Dylan M. Owen

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Tyler, A.I.I., Clarke, J.A., Seddon, J.M., Law, R.V. (2015). Solid State NMR of Lipid Model Membranes. In: Owen, D. (eds) Methods in Membrane Lipids. Methods in Molecular Biology, vol 1232. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1752-5_17

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  • DOI: https://doi.org/10.1007/978-1-4939-1752-5_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1751-8

  • Online ISBN: 978-1-4939-1752-5

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