Abstract
The development of boronic probes enabled reliable detection and quantitative analysis of hydrogen peroxide and peroxynitrite. The major product, in which boronate moiety of the probe is replaced by the hydroxyl group, is however common for both oxidants. Here, we describe how ortho-isomer of mitochondria-targeted phenylboronic acid can be used to detect and differentiate peroxynitrite-dependent and peroxynitrite-independent probe oxidation. This method highlights the detection and quantification of both the major, phenolic product and the minor, peroxynitrite-specific nitrated product of probe oxidation.
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Acknowledgments
This work was supported by NIH grant R01 HL063119 (B.K.). Support from a grant coordinated by JCET, No. POIG.01.01.02-00-069/09 (supported by the European Union from the resources of the European Regional Development Fund under the Innovative Economy Programme), is acknowledged. A.S. was supported by a grant IP2011 049271 from the Ministry of Science and Higher Education within the “Iuventus Plus” program.
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Zielonka, J., Sikora, A., Adamus, J., Kalyanaraman, B. (2015). Detection and Differentiation Between Peroxynitrite and Hydroperoxides Using Mitochondria-Targeted Arylboronic Acid. In: Weissig, V., Edeas, M. (eds) Mitochondrial Medicine. Methods in Molecular Biology, vol 1264. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2257-4_16
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DOI: https://doi.org/10.1007/978-1-4939-2257-4_16
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