Abstract
In vitro culture of neonatal murine cardiomyocytes is vital for understanding the functions of the heart. Cardiomyocyte cultures are difficult to maintain because they do not proliferate after birth. The maintenance of primary cultures of viable and functional cardiomyocytes is considerably affected by the yield from initial steps of isolation procedures. This protocol describes an efficient and rapid method for isolation and maintenance of long-term cultures of neonatal murine cardiomyocytes by effectively shortening the trypsin enzyme digestion period and the cardiomyocyte enrichment step.
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Acknowledgments
This work is supported by grants to R.S.V. by the Ministry of Human Resource Development (MHRD—BIO/2005–2006/007/MHRD/RAMS/859) and Department of Biotechnology, Ministry of Science and Technology (DBT-BT/PR5392/MED/14/693/2004).
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Parameswaran, S., Santhakumar, R., Vidyasekar, P., Verma, R.S. (2015). Enrichment of Cardiomyocytes in Primary Cultures of Murine Neonatal Hearts. In: Skuse, G., Ferran, M. (eds) Cardiomyocytes. Methods in Molecular Biology, vol 1299. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2572-8_2
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DOI: https://doi.org/10.1007/978-1-4939-2572-8_2
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2571-1
Online ISBN: 978-1-4939-2572-8
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