Editors:
- Includes cutting-edge methods and protocols for the study of coronaviruses in domestic animals and poultry
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Springer Protocols Handbooks (SPH)
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Table of contents (19 protocols)
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Front Matter
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Introduction
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Front Matter
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Conventional Technologies
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Front Matter
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Reverse Genetics
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Front Matter
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Molecular Diagnostics
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Front Matter
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About this book
This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Animal Coronaviruses serves as an ideal reference for researchers examining a wide variety of coronavirus species in the Coronaviridae.Reviews
“This book is unique in presenting current technologies available for detection of animal coronaviruses and research especially for diagnostic methodologies. The intention is to provide specific protocols and methods for individual coronaviruses, both conventional approaches and state-of-the art technologies. … The primary audience is diagnosticians, interns, and residents who work in the laboratory, but graduate students and researchers will benefit as well. … The book is essential for researchers and diagnosticians in veterinary virology.” (Dongwan Yoo, Doody's Book Reviews, June, 2016)
Editors and Affiliations
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Animal Disease Diagnostic Lab, Ohio Department of Agriculture, Reynoldsburg, USA
Leyi Wang
Bibliographic Information
Book Title: Animal Coronaviruses
Editors: Leyi Wang
Series Title: Springer Protocols Handbooks
DOI: https://doi.org/10.1007/978-1-4939-3414-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3412-6Published: 23 March 2016
Softcover ISBN: 978-1-4939-8044-4Published: 19 April 2018
eBook ISBN: 978-1-4939-3414-0Published: 22 March 2016
Series ISSN: 1949-2448
Series E-ISSN: 1949-2456
Edition Number: 1
Number of Pages: X, 218
Number of Illustrations: 10 b/w illustrations, 15 illustrations in colour
Topics: Infectious Diseases