Abstract
Imaging of the leukemic bone marrow microenvironment, also called the leukemic bone marrow niche, is an essential method to determine and to evaluate the progression of chronic myelogenous leukemia (CML) and other leukemias in murine models. In this chapter we introduce the murine model of CML primarily used in our laboratory by describing blood and bone marrow analysis as well as the method of histological sectioning and immunohistochemistry in combination with various stainings that can help to understand the complex interaction between leukemic cells, their normal hematopoietic counterparts, and the bone marrow microenvironment. We conclude with describing how to image the bone marrow niche using in vivo microscopy.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Deininger MW, Goldman JM, Melo JV (2000) The molecular biology of chronic myeloid leukemia. Blood 96:3343–3356
Vardiman JW, Thiele J, Arber DA, Brunning RD, Borowitz MJ, Porwit A et al (2009) The 2008 revision of the World Health Organization (WHO) classification of myeloid neoplasms and acute leukemia: rationale and important changes. Blood 114:937–951
Van Etten RA (2001) Models of chronic myeloid leukemia. Curr Oncol Rep 3:228–237
Krause DS, Scadden DT, Preffer FI (2013) The hematopoietic stem cell niche - home for friend and foe? Cytometry B Clin Cytom 84:7–20
Gallipoli P, Cook A, Rhodes S, Hopcroft L, Wheadon H, Whetton AD et al (2014) JAK2/STAT5 inhibition by nilotinib with ruxolitinib contributes to the elimination of CML CD34+ cells in vitro and in vivo. Blood 124:1492–1501
Koschmieder S, Göttgens B, Zhang P, Iwasaki-Arai J, Akashi K, Kutok JL et al (2005) Inducible chronic phase of myeloid leukemia with expansion of hematopoietic stem cells in a transgenic model of BCR-ABL leukemogenesis. Blood 105:324–334
Daley GQ, Van Etten RA, Baltimore D (1990) Induction of chronic myelogenous leukemia in mice by the P210bcr/abl gene of the Philadelphia chromosome. Science 247:824–830
Li S, Ilaria RL, Million RP, Daley GQ, Van Etten RA (1999) The P190, P210, and P230 forms of the BCR/ABL oncogene induce a similar chronic myeloid leukemia-like syndrome in mice but have different lymphoid leukemogenic activity. J Exp Med 189:1399–1412
Mulisch M, Welsch U (2010) Romeis Mikroskopische Technik. Springer Spektrum, Akademischer Verlag, Heidelberg/Berlin, pp 181–193
Welsch U (2010) Lehrbuch histologie. München, Urban & Fischer Verlag/Elsevier GmbH, pp 2–198
Van Hecke D (2002) Routine immunohistochemical staining today: choices to make, challenges to take. J Histotechnol 25:45–54
Ramos-Vara JA (2005) Technical aspects of immunohistochemistry. Vet Pathol 42:405–426
Schrimpf G (2002) Gentechnische Methoden - Eine Sammlung von Arbeitsanleitungen für das molekularbiologische Labor. Springer Spektrum, Akademischer Verlag GmbH, Heidelberg/Berlin, pp 327–351
Lo Celso C, Fleming HE, Wu JW, Zhao CX, Miake-Lye S, Fujisaki J et al (2009) Live-animal tracking of individual haematopoietic stem cells in their niche. Nature 457:92–96
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer Science+Business Media New York
About this protocol
Cite this protocol
Weissenberger, E.S., Krause, D.S. (2016). Histological and In Vivo Microscopic Analysis of the Bone Marrow Microenvironment in a Murine Model of Chronic Myelogenous Leukemia. In: Li, S., Zhang, H. (eds) Chronic Myeloid Leukemia. Methods in Molecular Biology, vol 1465. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-4011-0_6
Download citation
DOI: https://doi.org/10.1007/978-1-4939-4011-0_6
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-4009-7
Online ISBN: 978-1-4939-4011-0
eBook Packages: Springer Protocols