Abstract
Proteins play many roles in regulating the biochemistry of cells and therefore in the maintenance of good health. Though the genome contains essential information, it is at the translational and post-translational level that many other vital mechanisms of cellular control are to be found. Proteins exhibit a wide range of chemical properties and are involved in many aspects of biological systems, e.g., structural, enzymic catalysis, hormonal and other signalling processes. It is important to both identify and quantify the proteins that are key to understanding a particular pathology. In this chapter the methods used to achieve this are described.
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Abbreviations
- 2DPAGE:
-
Two dimensional polyacrylamide gel electrophoresis
- CSF:
-
Cerebrospinal fluid
- CVF:
-
Cervico vaginal fluid
- DIGE:
-
Fluorescent difference gel electrophoresis
- ELISA:
-
Enzyme linked immunosorbent assay
- IHC:
-
Immunohistochemistry
- MALDI-MS:
-
Matrix assisted laser desorption ionization mass spectrometry
- PCR:
-
Polymerase chain reaction
- RIA:
-
Radioimmunoassay
- SDS-PAGE:
-
Sodium dodecyl sulphate polyacrylamide gel electrophoresis
- WB:
-
Western Blotting
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KA acknowledges and thanks the Rotary Club of Williamstown, Victoria, Australia for partial salary support through the RoCan program
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Ashman, K., Rice, G., Mitchell, M. (2016). Proteomics Methods. In: Lakhani, S., Fox, S. (eds) Molecular Pathology in Cancer Research. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-6643-1_10
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