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Simultaneous Measurement of Mitochondrial and Glycolytic Activity in Quiescent Muscle Stem Cells

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Muscle Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1556))

Abstract

Cellular metabolism has recently been identified as an important regulator of cell identity, with several adult stem cell populations having been observed to undergo a shift in metabolism during important changes in cell state, such as during the shift from quiescence to proliferation. In this chapter, a method to characterize the metabolism of quiescent skeletal muscle stem cells is presented. This technique will allow for the comparison of quiescent muscle stem cells isolated from two or more different mouse models.

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Author information

Authors and Affiliations

  1. Stem Cell Metabolism & Regenerative Medicine Group, Basic & Clinical Myology Laboratory, Department of Physiology, The University of Melbourne, Parkville, VIC, 3010, Australia

    James G. Ryall Ph.D.

Authors
  1. James G. Ryall Ph.D.
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Correspondence to James G. Ryall Ph.D. .

Editor information

Editors and Affiliations

  1. Cell Biology Group, Department of Experimental and Health Sciences, University Pompeu Fabra (UPF), Barcelona, Spain

    Eusebio Perdiguero

  2. Division of Biological Sciences and Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO, USA

    DDW Cornelison

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Ryall, J.G. (2017). Simultaneous Measurement of Mitochondrial and Glycolytic Activity in Quiescent Muscle Stem Cells. In: Perdiguero, E., Cornelison, D. (eds) Muscle Stem Cells. Methods in Molecular Biology, vol 1556. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-6771-1_13

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  • DOI: https://doi.org/10.1007/978-1-4939-6771-1_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-6769-8

  • Online ISBN: 978-1-4939-6771-1

  • eBook Packages: Springer Protocols

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