Abstract
Regulated efflux of iron from cells is a fundamental event in controlling the intracellular pool of labile iron. Imbalance to this pool can be detrimental to the cell either through impairment to metabolic pathways when deficient or production of hydroxyl radicals when in excess. While ferroportin is currently the only known iron export pore protein in all cell types of the brain, its functional location is established through protein complexes that vary between cell types. Here, we describe selected experimental techniques that can evaluate iron flux as well as the downstream changes to the labile intracellular pool of iron within the whole brain or select cell types. Our aim is to provide the reader with previously applied procedures using resources available in most biochemical laboratories for interrogating cellular location and movement of iron in cells and tissue that has derived from brain.
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Wong, B.X., Lam, L.Q., Tsatsanis, A., Duce, J.A. (2017). Evaluating Iron Flux in the Brain. In: White, A. (eds) Metals in the Brain. Neuromethods, vol 124. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6918-0_10
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DOI: https://doi.org/10.1007/978-1-4939-6918-0_10
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