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Imaging Metals in the Brain by Laser Ablation–Inductively Coupled Plasma-Mass Spectrometry

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Metals in the Brain

Part of the book series: Neuromethods ((NM,volume 124))

Abstract

Laser ablation–inductively coupled plasma-mass spectrometry (LA-ICP-MS) has been an invaluable tool for accurate and precise measurement of metals in geological and environmental samples. However, like many advanced analytical techniques its versatility has seen its translation into several other disciplines, including neuroimaging. In this chapter, we discuss the principles of LA-ICP-MS technology and how it is applied to mesoscale imaging of metals in neurological tissue; evaluate the various approaches available for image construction and analysis; discuss the challenges and opportunities for quantitative metal imaging; and present a selection of applications demonstrating the power of this analytical technique as a tool for assessing both spatial distribution and absolute levels of essential metals in the brain.

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Authors and Affiliations

  1. Elemental Bio-imaging Facility, University of Technology Sydney, 123, Broadway, NSW, 2007, Australia

    Dominic J. Hare & Philip A. Doble

  2. The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, VIC, Australia

    Dominic J. Hare & Bence Paul

  3. School of Earth Sciences, The University of Melbourne, Parkville, VIC, Australia

    Bence Paul

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  1. Dominic J. Hare
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  2. Bence Paul
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  3. Philip A. Doble
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Correspondence to Philip A. Doble .

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  1. Department of Pathology, University of Melbourne, Melbourne, Victoria, Australia

    Anthony R. White

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Hare, D.J., Paul, B., Doble, P.A. (2017). Imaging Metals in the Brain by Laser Ablation–Inductively Coupled Plasma-Mass Spectrometry. In: White, A. (eds) Metals in the Brain. Neuromethods, vol 124. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6918-0_3

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  • DOI: https://doi.org/10.1007/978-1-4939-6918-0_3

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