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Stem Cell Banking pp 151–164Cite as

Cryobanking Pluripotent Stem Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1590))

Abstract

Cryobanking human pluripotent stem cells (hPSCs), be they human embryonic (hESCs) or induced pluripotent stem cells (iPSCs), is essential for their use in research and cell-based therapeutics. Working and master cell banks can be generated with a desired level of quality assurance applied during cell freezing and storage. Conventional vitrification has evolved to more advanced control rate freezing, culminating in a myriad of published protocols with variable proficiencies and clinical efficacies. Notwithstanding, standardized and reliable protocols are necessary for basic science through to applied research and clinical product development. This chapter details several methods for hPSC cryopreservation, suitable for routine application, high-quality research, and adaptable for clinical compliance.

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References

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Author information

Authors and Affiliations

  1. ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Fairy Meadow, New South Wales, 2519, Australia

    Jeremy M. Crook & Eva Tomaskovic-Crook

  2. Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, New South Wales, 2522, Australia

    Jeremy M. Crook & Eva Tomaskovic-Crook

  3. Department of Surgery, St Vincent’s Hospital, The University of Melbourne, Fitzroy, Victoria, 3065, Australia

    Jeremy M. Crook

  4. WiCell Research Institute, Madison, WI, USA

    Tenneille E. Ludwig

Authors
  1. Jeremy M. Crook
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  2. Eva Tomaskovic-Crook
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  3. Tenneille E. Ludwig
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Corresponding author

Correspondence to Jeremy M. Crook .

Editor information

Editors and Affiliations

  1. ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Squires Way, Fairy Meadow, New South Wales, Australia

    Jeremy M. Crook

  2. WiCell Research Institute, Madison, Wisconsin, USA

    Tenneille E. Ludwig

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Crook, J.M., Tomaskovic-Crook, E., Ludwig, T.E. (2017). Cryobanking Pluripotent Stem Cells. In: Crook, J., Ludwig, T. (eds) Stem Cell Banking. Methods in Molecular Biology, vol 1590. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6921-0_11

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  • DOI: https://doi.org/10.1007/978-1-4939-6921-0_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6919-7

  • Online ISBN: 978-1-4939-6921-0

  • eBook Packages: Springer Protocols

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