Abstract
The discovery and study of human neural stem cells has advanced our understanding of human neurogenesis, and the development of novel therapeutics based on neural cell replacement. Here, we describe methods to culture and cryopreserve human neural stem cells (hNSCs) for expansion and banking. Importantly, the protocols ensure that the multipotency of hNSCs is preserved to enable differentiation to neurons and supporting neuroglia.
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References
Crook JM, Kobayashi NR (2008) Human stem cells for modeling neurological disorders: accelerating the drug discovery pipeline. J Cell Biochem 105:1361–1366
Kobayashi N, Sui L, Tan PSL et al (2010) Modelling disrupted-inschizophrenia 1 loss of function in human neural progenitor cells: tools for molecular studies of human neurodevelopment and neuropsychiatric disorders. Mol Psychiatry 15:672–675
Acknowledgment
The authors wish to acknowledge funding from the Australian Research Council (ARC) Centre of Excellence Scheme (CE140100012).
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Crook, J.M., Tomaskovic-Crook, E. (2017). Culturing and Cryobanking Human Neural Stem Cells. In: Crook, J., Ludwig, T. (eds) Stem Cell Banking. Methods in Molecular Biology, vol 1590. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6921-0_15
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DOI: https://doi.org/10.1007/978-1-4939-6921-0_15
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6919-7
Online ISBN: 978-1-4939-6921-0
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