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Stem Cell Banking pp 115–129Cite as

Derivation of Human Embryonic Stem Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1590))

Abstract

Embryonic stem cells (ESCs) represent a mainstay for pluripotent stem cell research and development (R&D) and provide tangible opportunities for clinical translation including cell therapies and drug discovery. Moreover, in spite of the discovery of induced pluripotent stem cells (iPSCs), ESCs are an essential reference point, against which other pluripotent cells are compared. Hence, there is an ongoing need to derive and bank quality-controlled research-grade and clinical-grade ESC lines using established and standardized methods. Here, we provide a concise, step-by-step protocol for the derivation of ESCs from human embryos. While largely based on previously reported method for clinical-grade human ESC (hESC) line derivation, the protocol is suitable for routine application, although adaptable for clinical-compliance.

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References

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Acknowledgment

J.M.C. acknowledges funding from the Australian Research Council (ARC) Centre of Excellence Scheme (CE140100012).

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Authors and Affiliations

  1. ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Fairy Meadow, New South Wales, 2519, Australia

    Jeremy M. Crook

  2. Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, New South Wales, 2522, Australia

    Jeremy M. Crook

  3. Department of Surgery, St Vincent’s Hospital, The University of Melbourne, Fitzroy, Victoria, 3065, Australia

    Jeremy M. Crook

  4. Centre for Blood Cell Therapies, Peter MacCallum Cancer Centre, East Melbourne, Australia

    Lucy Kravets

  5. Genea Biomedx, Level 2, 321 Kent Street, Sydney, NSW, 2000, Australia

    Teija Peura

  6. Department of Medicine, Division of Endocrinology and Stem Cell Institute, McGuire Translational Research Facility, University of Minnesota, 2001 6th Street SE, Mail Code 2873, Minneapolis, MN, 55455, USA

    Meri T. Firpo

Authors
  1. Jeremy M. Crook
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  2. Lucy Kravets
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  3. Teija Peura
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  4. Meri T. Firpo
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Corresponding author

Correspondence to Jeremy M. Crook .

Editor information

Editors and Affiliations

  1. ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Squires Way, Fairy Meadow, New South Wales, Australia

    Jeremy M. Crook

  2. WiCell Research Institute, Madison, Wisconsin, USA

    Tenneille E. Ludwig

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Crook, J.M., Kravets, L., Peura, T., Firpo, M.T. (2017). Derivation of Human Embryonic Stem Cells. In: Crook, J., Ludwig, T. (eds) Stem Cell Banking. Methods in Molecular Biology, vol 1590. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6921-0_8

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  • DOI: https://doi.org/10.1007/978-1-4939-6921-0_8

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6919-7

  • Online ISBN: 978-1-4939-6921-0

  • eBook Packages: Springer Protocols

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