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Stoichiometry and Turnover of the Stator and Rotor

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The Bacterial Flagellum

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1593))

Abstract

Fluorescence imaging techniques using green fluorescent protein (GFP) and related fluorescent proteins are utilized to monitor and analyze a wide range of biological processes in living cells. Stepwise photobleaching experiments can determine the stoichiometry of protein complexes. Fluorescence recovery after photobleaching (FRAP) experiments can reveal in vivo dynamics of biomolecules. In this chapter, we describe methods to detect the subcellular localization, stoichiometry, and turnovers of stator and rotor components of the Salmonella flagellar motor.

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Acknowledgments

We thank Keiichi Namba, Nobunori Kami-ike, and Masahiro Ueda for continuous support and encouragement. This research has been supported in part by JSPS KAKENHI Grant Numbers JP15K14498 and JP15H05593 (to Y.V.M.) and JP26293097 (to T.M.) and MEXT KAKENHI Grant Numbers JP15H01335 (to Y.V.M.) and JP25121718 (to T.M.).

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Authors and Affiliations

  1. Quantitative Biology Center, RIKEN, 6-2-3 Furuedai, Suita, Osaka, 565-0874, Japan

    Yusuke V. Morimoto

  2. Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Tohru Minamino

Authors
  1. Yusuke V. Morimoto
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  2. Tohru Minamino
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Corresponding author

Correspondence to Yusuke V. Morimoto .

Editor information

Editors and Affiliations

  1. Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan

    Tohru Minamino

  2. Quantitative Biology Center RIKEN, Suita, Osaka, Japan

    Keiichi Namba

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Morimoto, Y.V., Minamino, T. (2017). Stoichiometry and Turnover of the Stator and Rotor. In: Minamino, T., Namba, K. (eds) The Bacterial Flagellum. Methods in Molecular Biology, vol 1593. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6927-2_16

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  • DOI: https://doi.org/10.1007/978-1-4939-6927-2_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6926-5

  • Online ISBN: 978-1-4939-6927-2

  • eBook Packages: Springer Protocols

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