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Flow Cytometric Detection of Circulating Tumor Cells Using a Candidate Stem Cell Marker, p75 Neurotrophin Receptor (p75NTR)

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Circulating Tumor Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1634))

Abstract

The most widely studied detection for circulating tumor cells (CTCs) in peripheral blood of cancer patients has been based on immunomagnetic enrichment using antibodies against epithelial cell adhesion molecule (EpCAM), which is overexpressed in epithelial cells. A neurotrophin receptor p75 (p75NTR) is expressed in a candidate stem cell fraction in esophageal squamous cell carcinoma (ESCC), which shows significantly higher colony formation, enhanced tumor formation in mice, along with strong expression of epithelial mesenchymal transition-related genes. Here, we describe a method to detect CTCs in ESCC based on the combined expression of EpCAM and p75NTR using flow cytometry, demonstrating the feasibility of expression analysis of multiple cell surface markers in viable cells.

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Acknowledgment

This work was supported by JSPS KAKENHI Grant Number JP15K10088.

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Authors and Affiliations

  1. Department of Surgery and Science, Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, 2630 Sugitani, Toyama City, Toyama, 930-0194, Japan

    Tomoyuki Okumura M.D., Ph.D., Tetsuji Yamaguchi, Toru Watanabe & Takuya Nagata

  2. Department of Nanobio Drug Discovery, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida-Shimo-Adachi-cho, Sakyo-ku, Kyoto, 606-8501, Japan

    Yutaka Shimada

Authors
  1. Tomoyuki Okumura M.D., Ph.D.
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  2. Tetsuji Yamaguchi
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  3. Toru Watanabe
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  4. Takuya Nagata
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  5. Yutaka Shimada
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Corresponding author

Correspondence to Tomoyuki Okumura M.D., Ph.D. .

Editor information

Editors and Affiliations

  1. Division of Hematology Oncology, Helen Diller Family Comprehensive Cancer Center, University of California-San Francisco, San Francisco, California, USA

    Mark Jesus M. Magbanua

  2. Division of Hematology Oncology, Helen Diller Family Comprehensive Cancer Center, University of California-San Francisco, San Francisco, California, USA

    John W. Park

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Okumura, T., Yamaguchi, T., Watanabe, T., Nagata, T., Shimada, Y. (2017). Flow Cytometric Detection of Circulating Tumor Cells Using a Candidate Stem Cell Marker, p75 Neurotrophin Receptor (p75NTR). In: M. Magbanua, M., W. Park, J. (eds) Circulating Tumor Cells. Methods in Molecular Biology, vol 1634. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7144-2_18

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  • DOI: https://doi.org/10.1007/978-1-4939-7144-2_18

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7143-5

  • Online ISBN: 978-1-4939-7144-2

  • eBook Packages: Springer Protocols

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