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Measuring Mitochondrial Substrate Utilization in Skeletal Muscle Stem Cells

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Skeletal Muscle Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1668))

Abstract

Skeletal muscle stem cells (MuSCs) derived from the somatic mesoderm play a critical role in successful muscle regeneration following injury and trauma. MuSCs have been found to undergo rapid changes in metabolism following a change in cell state, such as that which occurs during the transition from quiescence to an actively proliferating state. There is mounting evidence that metabolism is critically important in the regulation of quiescence, activation, and differentiation and thus the development of new techniques that aim to further probe the metabolism of MuSCs is essential. The Seahorse XF Bioanalyzer is a powerful tool that simultaneously measures the extracellular rate of change in oxygen partial pressure and pH, providing a method to measure mitochondrial respiration and lactate production. In this chapter, we describe the use of key metabolic inhibitors that allow for the investigation of mitochondrial substrate utilization in primary MuSCs.

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Author information

Authors and Affiliations

  1. Stem Cell Metabolism and Regenerative Medicine Group, Basic and Clinical Myology Laboratory, The University of Melbourne, Parkville, VIC, 3010, Australia

    C. Hai Ly

  2. Stem Cell Metabolism and Regenerative Medicine Group, Basic and Clinical Myology Laboratory, Department of Physiology, School of Biomedical Sciences, The University of Melbourne, Melbourne, VIC, 3010, Australia

    James G. Ryall Ph.D.

Authors
  1. C. Hai Ly
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  2. James G. Ryall Ph.D.
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Corresponding author

Correspondence to James G. Ryall Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Physiology, The University of Melbourne, Parkville, Victoria, Australia

    James G. Ryall

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Ly, C.H., Ryall, J.G. (2017). Measuring Mitochondrial Substrate Utilization in Skeletal Muscle Stem Cells. In: Ryall, J. (eds) Skeletal Muscle Development. Methods in Molecular Biology, vol 1668. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7283-8_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7283-8_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7282-1

  • Online ISBN: 978-1-4939-7283-8

  • eBook Packages: Springer Protocols

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