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Ex Vivo Dual Perfusion of the Human Placenta: Disease Simulation, Therapeutic Pharmacokinetics and Analysis of Off-Target Effects

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Preeclampsia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1710))

Abstract

In recent years ex vivo dual perfusion of the human placental lobule is seeing an international renaissance in its application to understanding fetal health and development. Here, we discuss the methods and uses of this technique in the evaluation of (1) vascular function, (2) transplacental clearance, (3) hemodynamic and oxygenation changes associated with pregnancy complications on placental structure and function, and (4) placental toxicology and post-perfusion evaluation of tissue architecture.

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Author information

Authors and Affiliations

  1. Maternal and Fetal Health Research Centre, Division of Developmental Biology & Medicine, School of Medical Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Science Centre, Manchester, UK

    Paul Brownbill

  2. St. Mary’s Hospital, Central Manchester University Hospitals, NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK

    Paul Brownbill

  3. Institute of Child Health, University College London, London, UK

    Neil Sebire

  4. The Ritchie Centre, Hudson Institute of Medical Research, Clayton, VIC, Australia

    Erin V. McGillick, Stacey Ellery & Padma Murthi

  5. The Department of Obstetrics and Gynecology, School of Clinical Sciences, Monash University, Clayton, VIC, Australia

    Erin V. McGillick, Stacey Ellery & Padma Murthi

  6. Department of Medicine, School of Clinical Sciences, Monash University, Clayton, VIC, Australia

    Padma Murthi

Authors
  1. Paul Brownbill
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  2. Neil Sebire
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  3. Erin V. McGillick
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  4. Stacey Ellery
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  5. Padma Murthi
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Corresponding author

Correspondence to Paul Brownbill .

Editor information

Editors and Affiliations

  1. Monash Medical Centre, Monash University, Clayton, Victoria, Australia

    Padma Murthi

  2. INRS-Institut Armand-Frappier, Laval, Québec, Canada

    Cathy Vaillancourt

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Brownbill, P., Sebire, N., McGillick, E.V., Ellery, S., Murthi, P. (2018). Ex Vivo Dual Perfusion of the Human Placenta: Disease Simulation, Therapeutic Pharmacokinetics and Analysis of Off-Target Effects. In: Murthi, P., Vaillancourt, C. (eds) Preeclampsia . Methods in Molecular Biology, vol 1710. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7498-6_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7498-6_14

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7497-9

  • Online ISBN: 978-1-4939-7498-6

  • eBook Packages: Springer Protocols

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