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Isolation and Characterization of Mesenchymal Stem/Stromal Cells Derived from Human Third Trimester Placental Chorionic Villi and Decidua Basalis

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Book cover Preeclampsia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1710))

Abstract

The decidua basalis and placental chorionic villi are critical components of maternal-fetal interface, which plays a critical role in normal placental development. Failure to form a proper maternal-fetal interface is associated with clinically important placental pathologies including preeclampsia and fetal growth restriction. Placental trophoblast cells are well known for their critical roles in establishing the maternal-fetal interface; however accumulating evidence also implicates mesenchymal stem/stromal cells that envelop the maternal and fetal blood vessels as playing an important role in the formation and efficient functioning of the interface. Moreover, recent studies associate abnormal mesenchymal stem/stromal cell function in the development of preeclampsia. Further research is needed to fully understand the role that these cells play in this clinically important placental pathology.

The intimate relationship between maternal and fetal tissues at the interface poses significant problems in the enrichment of decidua basalis and chorionic villous mesenchymal stem/stromal cells without significant cross-contamination. The protocols described below for the enrichment and characterization of mesenchymal stem/stromal cells from the maternal-fetal interface produce highly enriched cells that conform to international standards and show minimal cross-contamination.

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Acknowledgments

We greatly acknowledge the work of Dr Rishika Anne Pace, Dr Sharon Qin, Melissa Duggan, and Anthony Borg who contributed to the optimization of these protocols.

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynaecology, Royal Women’s Hospital, University of Melbourne, Parkville, VIC, Australia

    Gina D. Kusuma & Bill Kalionis

  2. Department of Maternal-Fetal Medicine Pregnancy Research Centre, Royal Women’s Hospital, Parkville, VIC, Australia

    Gina D. Kusuma & Bill Kalionis

  3. Stem Cells and Regenerative Medicine Department, King Abdullah International Medical Research Center, College of Science and Health Professions, King Saud Bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, National Guard Health Affairs, P.O. Box 3660, Mail Code 3124, Riyadh, 11481, Kingdom of Saudi Arabia

    Mohamed H. Abumaree

  4. Victorian Clinical Genetics Services, Murdoch Children’s Research Institute, Royal Children’s Hospital, Parkville, VIC, Australia

    Mark D. Pertile

  5. Department of Paediatrics, Royal Children’s Hospital, University of Melbourne, Parkville, VIC, Australia

    Mark D. Pertile

Authors
  1. Gina D. Kusuma
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  2. Mohamed H. Abumaree
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  3. Mark D. Pertile
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  4. Bill Kalionis
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Corresponding author

Correspondence to Bill Kalionis .

Editor information

Editors and Affiliations

  1. Monash Medical Centre, Monash University, Clayton, Victoria, Australia

    Padma Murthi

  2. INRS-Institut Armand-Frappier, Laval, Québec, Canada

    Cathy Vaillancourt

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Kusuma, G.D., Abumaree, M.H., Pertile, M.D., Kalionis, B. (2018). Isolation and Characterization of Mesenchymal Stem/Stromal Cells Derived from Human Third Trimester Placental Chorionic Villi and Decidua Basalis. In: Murthi, P., Vaillancourt, C. (eds) Preeclampsia . Methods in Molecular Biology, vol 1710. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7498-6_19

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  • DOI: https://doi.org/10.1007/978-1-4939-7498-6_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7497-9

  • Online ISBN: 978-1-4939-7498-6

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