Abstract
Mass spectrometry imaging (MSI) is a developing technique to measure the spatiotemporal distribution of many biomolecules in tissues. Over the preceding decade MSI has been adopted by plant biologists and applied in a broad range of areas including: primary metabolism, natural products, plant defense, plant responses to abiotic and biotic stress, plant lipids, and the developing field of spatial metabolomics. This methods chapter covers preparation of plant tissues for matrix-assisted laser desorption ionization (MALDI)-MSI, including sample embedding and freezing, sectioning, mounting, and matrix deposition using both sublimation and spray deposition prior to MSI analysis.
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Acknowledgment
The University of Melbourne node of Metabolomics Australia, a National Collaborative Research Infrastructure Strategy (NCRIS) initiative under Bioplatforms Australia (BPA) Pty Ltd.
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Boughton, B.A., Thinagaran, D. (2018). Mass Spectrometry Imaging (MSI) for Plant Metabolomics. In: António, C. (eds) Plant Metabolomics. Methods in Molecular Biology, vol 1778. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7819-9_17
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DOI: https://doi.org/10.1007/978-1-4939-7819-9_17
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