Abstract
In vivo time-lapse microscopy provides important information about the kinetics of cellular events and their control by interactions with neighboring cells. Here, we describe the generation and use of transgenic zebrafish to visualize dynamics of myelinating glia using cell type-specific expression and microscopy of genetically encoded fluorescent proteins. With this method, we are able to simultaneously separate and trace up to three different colors over time.
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Acknowledgments
Our group is supported by an Emmy Noether fellowship of the German Research Foundation (DFG, Cz226/1-1), a Starting Grant of the European Research Council (ERC-StG 714440, MecMy) and the Munich Cluster of Systems Neurology (SyNergy, DFG, EXC1010).
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Vagionitis, S., Czopka, T. (2018). Visualization and Time-Lapse Microscopy of Myelinating Glia In Vivo in Zebrafish. In: Woodhoo, A. (eds) Myelin. Methods in Molecular Biology, vol 1791. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7862-5_3
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DOI: https://doi.org/10.1007/978-1-4939-7862-5_3
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7861-8
Online ISBN: 978-1-4939-7862-5
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