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Isolation and Characterization of Mononuclear Phagocytes in the Mouse Lung and Lymph Nodes

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Lung Innate Immunity and Inflammation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1809))

Abstract

There is a diverse population of mononuclear phagocytes (MPs) in the lungs, comprised of macrophages, dendritic cells (DCs), and monocytes. The existence of these various cell types suggests that there is a clear division of labor and delicate balance between the MPs under steady-state and inflammatory conditions. Here we describe how to identify pulmonary MPs using flow cytometry and how to isolate them via cell sorting. In steady-state conditions, murine lungs contain a uniform population of alveolar macrophages (AMs), three distinct interstitial macrophage (IM) populations, three DC subtypes, and a small number of tissue-trafficking monocytes. During an inflammatory response, the monocyte population is more abundant and complex since it acquires either macrophage-like or DC-like features. All in all, studying how these cell types interact with each other, structural cells, and other leukocytes within the environment will be important to understanding their role in maintaining homeostasis and during the development of disease.

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Abbreviations

AM:

Alveolar macrophage

BAL:

Bronchoalveolar lavage

DC:

Dendritic cell

IM:

Interstitial macrophage

IN

Intranasal

IT:

Intratracheal

LLN:

Mediastinal lung-draining lymph node

MP:

Mononuclear phagocyte

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Acknowledgment

Grant support: C.V.J. NIH R01 HL115334 and R01 HL135001.

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Authors and Affiliations

  1. Department of Pediatrics, National Jewish Health, Denver, CO, USA

    Sophie L. Gibbings & Claudia V. Jakubzick

  2. Department of Microbiology and Immunology, University of Colorado, Denver, CO, USA

    Claudia V. Jakubzick

Authors
  1. Sophie L. Gibbings
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  2. Claudia V. Jakubzick
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Corresponding author

Correspondence to Claudia V. Jakubzick .

Editor information

Editors and Affiliations

  1. Department of Immunology and Microbiology University of Colorado School of Medicine, Department of Biomedical Research and Center for Genes Environment and Health, National Jewish Health Denver, CO, USA, Aurora,, Colorado, USA

    Scott Alper

  2. Division of Pulmonary Sciences and Critical Care Medicine Department of Medicine University of Colorado Denver, Division of Pulmonary, Critical Care and Sleep Medicine Department of Medicine National Jewish Health Denver, CO, USA, Aurora, Colorado, USA

    William J. Janssen

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Gibbings, S.L., Jakubzick, C.V. (2018). Isolation and Characterization of Mononuclear Phagocytes in the Mouse Lung and Lymph Nodes. In: Alper, S., Janssen, W. (eds) Lung Innate Immunity and Inflammation. Methods in Molecular Biology, vol 1809. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8570-8_3

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  • DOI: https://doi.org/10.1007/978-1-4939-8570-8_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8569-2

  • Online ISBN: 978-1-4939-8570-8

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