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Programmed Necrosis pp 53–61Cite as

Methods for Studying TNF-Mediated Necroptosis in Cultured Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1857))

Abstract

Necroptosis is a caspase-independent form of programmed cell death that is induced by a variety of different signalling cascades—all culminating in the activation of the pseudokinase mixed lineage kinase domain-like (MLKL). TNF-induced necroptosis is the most intensively studied of these pathways. Here we describe reagents and cell-based techniques that can be used to investigate TNF-mediated necroptosis in the lab.

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Acknowledgment

We wish to acknowledge the Australian National Health and Medical Research Council (GNT1105023, GNT1107149 and GNT1142669) and the Melbourne International Research Scholarship for funding this work.

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Authors and Affiliations

  1. Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia

    Zikou Liu, John Silke & Joanne M. Hildebrand

  2. Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia

    Zikou Liu, John Silke & Joanne M. Hildebrand

Authors
  1. Zikou Liu
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  2. John Silke
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  3. Joanne M. Hildebrand
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Corresponding authors

Correspondence to John Silke or Joanne M. Hildebrand .

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Editors and Affiliations

  1. Precision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA

    Adrian T. Ting

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Liu, Z., Silke, J., Hildebrand, J.M. (2018). Methods for Studying TNF-Mediated Necroptosis in Cultured Cells. In: Ting, A. (eds) Programmed Necrosis. Methods in Molecular Biology, vol 1857. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8754-2_5

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  • DOI: https://doi.org/10.1007/978-1-4939-8754-2_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8753-5

  • Online ISBN: 978-1-4939-8754-2

  • eBook Packages: Springer Protocols

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