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Monitoring Dendritic Cell Activation and Maturation

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Antigen Processing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1988))

Abstract

Since the 1997 discovery that the first identified human homolog of Drosophila Toll could activate the innate immune system, the innate arm of immunity has rapidly taken on a new light as an important player in the recognition of pathogens and damaged self. The recognition of danger by dendritic cells (DC) is a crucial step in activating the adaptive immune system. Different DC express varied subsets of pattern recognition receptors (PRR), enabling both overlap and exclusivity in the recognition of danger signals by DC. PRR-mediated DC maturation and activation can be measured by changes in the surface expression of costimulatory as well as coinhibitory molecules, changes in size and shape of the DC and by their production of different cytokines.

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Acknowledgments

This work was supported by an NHMRC Senior Research Fellowship 1077633 (M.O.K.), and NHMRC Project grants 1085934; 1002903 to M.O.K. and K.R.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology & Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia

    Christophe Macri & Meredith O’Keeffe

  2. Peter MacCallum Cancer Centre, Melbourne, VIC, Australia

    Ben Fancke

  3. Mater Research, The University of Queensland, Woolloongabba, QLD, Australia

    Kristen J. Radford

Authors
  1. Christophe Macri
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  2. Ben Fancke
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  3. Kristen J. Radford
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  4. Meredith O’Keeffe
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Corresponding author

Correspondence to Meredith O’Keeffe .

Editor information

Editors and Affiliations

  1. INSERM U1151, Université Paris Descartes, Hopital Necker-Enfants Malades, Paris, France

    Peter van Endert

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Macri, C., Fancke, B., Radford, K.J., O’Keeffe, M. (2019). Monitoring Dendritic Cell Activation and Maturation. In: van Endert, P. (eds) Antigen Processing. Methods in Molecular Biology, vol 1988. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9450-2_28

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  • DOI: https://doi.org/10.1007/978-1-4939-9450-2_28

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9449-6

  • Online ISBN: 978-1-4939-9450-2

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