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Formulation of Drosophila Food for Various Feeding Studies

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Fundamental Approaches to Screen Abnormalities in Drosophila

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Drosophila melanogaster serves as an important model organism in the fields of genetics and developmental biology. Recent studies have indicated the use of Drosophila in a number of other studies such as metabolic, nanotoxicological and immunological studies. Rearing of fly in the laboratory condition is an important task to work with this organism. Different culture media are developed to maintain the growth and development of the flies where the components of the food play important roles. Drosophila used in various toxicological or metabolic studies often involve oral intake of various nanoparticles, pathogens, antibiotics and molecules to study metabolism, and this is done by mixing the compounds with fly food in proper concentration. This chapter describes the modification of fly food with various treatments such as the addition of nanoparticles, bacteria, antibiotics and AGE compounds. These protocols will help to use fly for various feeding experiments.

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Acknowledgements

SP and SM are thankful to MHRD for financial support. MM lab is supported by Grant No. BT/PR21857/NNT/28/1238/2017, EMR/2017/003054, Odisha DBT 3325/ST(BIO)-02/2017.

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Author notes

  1. Subhashree Priyadarsini and Sumit Mukherjee contributed equally with all other contributors.

Authors and Affiliations

  1. Neural Developmental Biology Lab, Department of Life Science, National Institute of Technology Rourkela, Rourkela, Odisha, India

    Subhashree Priyadarsini, Sumit Mukherjee & Monalisa Mishra

Authors
  1. Subhashree Priyadarsini
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  2. Sumit Mukherjee
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  3. Monalisa Mishra
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Corresponding author

Correspondence to Monalisa Mishra .

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Editors and Affiliations

  1. Neural Developmental Biology Lab, Department of Life Science, National Institute of Technology Rourkela, Rourkela, Odisha, India

    Monalisa Mishra

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Priyadarsini, S., Mukherjee, S., Mishra, M. (2020). Formulation of Drosophila Food for Various Feeding Studies. In: Mishra, M. (eds) Fundamental Approaches to Screen Abnormalities in Drosophila. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-9756-5_1

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  • DOI: https://doi.org/10.1007/978-1-4939-9756-5_1

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-9755-8

  • Online ISBN: 978-1-4939-9756-5

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