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Assessing the Role of MIF in Plasmodium spp. Infections Using Ex Vivo Models

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Macrophage Migration Inhibitory Factor

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2080))

Abstract

Ex vivo techniques are a valuable tool for the investigation of how immune cells respond to Plasmodium spp. antigen, allowing examination of various aspects of the immune response under controlled conditions. Here we describe how to isolate peripheral blood mononuclear cells (PBMC) from donors and coculture them with purified P. falciparum-infected red blood cells (iRBC) to investigate the role of MIF during Plasmodium spp. infection.

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Author information

Authors and Affiliations

  1. Department of Medicine (RMH), Peter Doherty Institute, University of Melbourne, Melbourne, VIC, Australia

    Elizabeth H. Aitken

Authors
  1. Elizabeth H. Aitken
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Corresponding author

Correspondence to Elizabeth H. Aitken .

Editor information

Editors and Affiliations

  1. Rheumatology Group, Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences at Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash Medical Centre, Monash University, Clayton, VIC, Australia

    James Harris

  2. Rheumatology Group, Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences at Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash Medical Centre, Monash University, Clayton, VIC, Australia

    Eric F. Morand

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Aitken, E.H. (2020). Assessing the Role of MIF in Plasmodium spp. Infections Using Ex Vivo Models. In: Harris, J., Morand, E. (eds) Macrophage Migration Inhibitory Factor. Methods in Molecular Biology, vol 2080. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9936-1_16

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  • DOI: https://doi.org/10.1007/978-1-4939-9936-1_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9935-4

  • Online ISBN: 978-1-4939-9936-1

  • eBook Packages: Springer Protocols

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