Abstract
A cellulase production process was developed by growing the fungi Trichoderma reesei and Aspergillus phoenicis on dairy manure. T. reesei produced a high total cellulase titer (1.7 filter paper units [FPU]/mL, filter paper activity) in medium containing 10 g/L of manure (dry basis [w/w]), 2 g/L KH2PO4, 2 mL/L of Tween-80, and 2mg/L of CoCl2. However, β-glucosidase activity in the T. reesei-enzyme system was very low. T. reesei was then cocultured with A. phoenicis to enhance the β-glucosidase level. The mixed culture resulted in a relatively high level of total cellulase (1.54 FPU/mL) and β-glucosidase (0.64 IU/mL). The ratio of β-glucosidase activity to filter paper activity was 0.41, suitable for hydrolyzing manure cellulose. The crude enzyme broth from the mixed culture was used for hydrolyzing the manure cellulose, and the produced glucose was significantly (p < 0.01) higher than levels obtained by using the commercial enzyme or the enzyme broth of the pure culture T. reesei.
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References
Fontenot, J. P., Smith, L. W., and Sutton, A. L. (1983), J. Anim. Sri. 57(Suppl. 2), 221.
Moller, H. B., Sommer, S. G., and Ahring, B. K. (2002), Bioresour. Technol. 85, 189–196.
Sun, Y. and Cheng, J. (2002), Bioresour. Technol. 83, 1–11.
von Sivers, M. and Zacchi, G. (1995), Bioresour. Technol. 51, 43–52.
Wen, Z., Liao, W., and Chen, S. (2004), Bioresour. Technol. 91, 31–39.
Duff, S. J. B. and Murray, W. D. (1996), Bioresour. Technol. 55, 1–33.
Reczey, K., Szengyel, Z., Eklund, R., and Zacchi, G. (1996), Bioresour. Technol. 57, 25–30.
Ju, L. K. and Afolabi, O. A. (1999), Biotechnol. Prog. 15, 91–97.
Rajoka, M. I. and Malik, K. A. (1997), Bioresour. Technol. 59, 21–27.
Ogel, Z. B., Yarangumeli, K., Dundar, H., and Ifrij, I. (2001), Enzyme Micro. Technol. 28, 689–695.
Romero, M. D., Aguado, J. Gonzalez, L., and Ladero, M. (1999), Enzyme Micro. Technol. 25, 244–250.
Kalogeris, E., Christakopoulos, P., Katapodis, P., Alexiou, A., Vlachou, S., Kekos, D., and Macris, B.J. (2003), Process Biochem. 38, 1099–1104.
Xia, L. and Cen, P. (1999), Process Biochem. 34, 909–912.
Smits, J. P., Rinzema A., Tramper, J., van Sonsbeek, H. M., and Knol, W. (1996), Appl. Microbol Biotechnol. 46, 489–496.
Umikalsom, M. S., Ariff, A. B., Shamsuddin, Z. H., Tong, C. C, Hassan, M. A., and Karim, M. I. A. (1997), Appl. Microbiol. Biotechnol. 47, 590–595.
Haddadin, M. S. Y., Abu-Reesh, I. M., Haddadin. F. A. S.. and Robinson, R. K. (2001), Biores. Technol. 78, 225–230.
Mandel, M. and Weber, J. (1969), Adv. Chem. Ser. 95, 391–414.
Goering, H.K. and Van Soest, P.J. (1970), in Agricultural Handbook No. 379, Agricultural Research Service, US Department of Agriculture, Washington, DC, pp. 1–20.
Eaton, A. D., Clesceri, L. S., and Greengerg, A. E. (1995), Standard Methods for the Examination of Water and Wastewater, 19th Ed., American Public Health Association, Washington, DC.
Ghose, T. K. (1987), Pure Appl. Chem. 59, 257–268.
Umikalsom, M. S., Ariff, A. B., Zulkifli, H. S., Tong, C. C, Hassan M. A., and Karim, M. I. A. (1997) Bioresour. Technol. 62, 1–9.
Henze, M., Harremoes, P., Jansen, J. L. C, and Arvin, E. (1996). Wastewater Treatment: Biological and Chemical Process, 2nd Ed., Springer, Berlin.
Tangnu, S. K., Blanch, H. W., and Wilke, C. R. (1981), Biotechnol. Bioeng. 23, 1837–1849.
Cochet, N. (1991), Enzyme Micro. Technol. 13, 104–109.
Duff, S. J. B., Cooper, D. G., and Fuller, O. M, (1987), Enzyme Micro. Technol. 9, 47–52.
Duenas, R., Tengerdy, R. P., and Gutierrez-Correa, M. (1995), World. J. Microbiol Biotechnol. 11, 333–337.
Gutierrez-Correa, M. and Tengerdy, R.P. (1997), Biotechnol. Lett. 19, 665–667.
Flachner, B., Brumbauer, A., and Reczey, K. (1999), Enzyme Micro. Technol. 24, 362–367.
Gutierrez-Correa, M, Portal, L., Moreno, P., and Tengerdy, R. P. (1999), Bioresour. Technol. 68, 173–178.
Massadeh, M. I., Yusoff, W. M. W., Omar, O., and Kader, J. (2001), Biotechnol. Lett. 23, 1771–1774.
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Wen, Z., Liao, W., Chen, S. (2005). Production of Cellulase/β-Glucosidase by the Mixed Fungi Culture of Trichoderma reesei and Aspergillus phoenicis on Dairy Manure. In: Davison, B.H., Evans, B.R., Finkelstein, M., McMillan, J.D. (eds) Twenty-Sixth Symposium on Biotechnology for Fuels and Chemicals. ABAB Symposium. Humana Press. https://doi.org/10.1007/978-1-59259-991-2_9
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DOI: https://doi.org/10.1007/978-1-59259-991-2_9
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