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Neural Differentiation of Human Embryonic Stem Cells

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Book cover Neural Stem Cells

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 438))

Summary

Embryonic stem cells (ESCs) are pluripotent and capable of indefinite self-renewal in vitro. These features make them a highly advantageous source for deriving any cell type of the central and peripheral nervous system. We describe neural induction of human (h)ESCs, by using the bone morphogenic protein inhibitor protein noggin. Neural progenitors derived from noggin-treated hESCs can be propagated as neurospheres and further differentiated in vitro and in vivo to mature neurons and glia. This complete protocol of neural differentiation, from hESCs to mature neuronal cells, can be used as an in vitro model to study human neurogenesis and neurodegeneration.

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Author information

Authors and Affiliations

  1. Monash Institute of Medical Research and the Australian Stem Cell Centre, Clayton, Victoria, Australia

    Mirella Dottori phD

  2. Centre for Neuroscience and Department of Pharmacology, The University of Melbourne, Parkville, Victoria, Australia

    Mirella Dottori phD

  3. Center for Stem Cell and Regenerative Medicine, Keck School of Medicine of the University of Southern California, Los Angeles, CA

    Martin F. Pera phD

Authors
  1. Mirella Dottori phD
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  2. Martin F. Pera phD
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Editor information

Editors and Affiliations

  1. Keck School of Medicine, University of Southern California, 2025 Zonal Ave., Los Angeles, CA 90033, USA

    Leslie P. Weiner

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Dottori, M., Pera, M.F. (2008). Neural Differentiation of Human Embryonic Stem Cells. In: Weiner, L.P. (eds) Neural Stem Cells. Methods in Molecular Biology™, vol 438. Humana Press. https://doi.org/10.1007/978-1-59745-133-8_3

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  • DOI: https://doi.org/10.1007/978-1-59745-133-8_3

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-846-1

  • Online ISBN: 978-1-59745-133-8

  • eBook Packages: Springer Protocols

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